Organotypic culture of fetal rat lung: evaluation and comparison with organ culture.
Organotypic culture of fetal rat lung: evaluation and comparison with organ culture.
复制标题
胎鼠肺的器官型培养：与器官培养的评估和比较。
DOI：
10.1164/arrd.1981.123.3.313
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Lindsey,S
中科院分区：
文献类型：
--
作者：
Gross,I;Freedman,RM;Wilson,CM;Lindsey,S
19 天胎鼠肺的单分散悬浮液铺在明胶海绵上时重新聚集形成肺泡状结构。这些结构几乎完全由肺泡 II 型细胞组成，而整个培养物含有约 65% II 型细胞。培养 8 天后，器官型培养物的磷脂含量和组成与在器官培养物中生长 48 小时的 19 天胎鼠肺外植体非常相似，胆碱和乙酸盐掺入磷脂的模式也是如此。器官型培养物分泌到培养基中的磷脂酰胆碱非常少。初步观察表明，器官培养物对激素更敏感。对于研究激素对胎儿肺成熟的影响，器官型培养似乎并没有比更简单和更快的器官培养提供任何主要优势。它们可能有助于检查 II 型细胞的代谢和合成途径。
Monodispersed suspensions of 19-day fetal rat lung when plated onto gelatin sponges reaggregated to form alveolarlike structures. These structures consisted almost entirely of alveolar type II cells, whereas the whole cultures contained about 65% type II cells. The phospholipid content and composition of the organotypic cultures after 8 days of incubation was very similar to that of explants of 19-day fetal rat lung grown in organ culture for 48 h, as was the pattern of incorporation of choline and acetate into phospholipids. Very little phosphatidylcholine was secreted by the organotypic cultures into the culture medium. Initial observations suggested that the organ cultures are more responsive to hormones. Organotypic cultures do not appear to offer any major advantage over the simpler and quicker organ cultures for studies of hormonal influences on fetal lung maturation. They may be useful for examining the metabolism and synthetic pathways of the type II cell.