Mononuclear muscle cells in Drosophila ovaries revealed by GFP protein traps
Mononuclear muscle cells in Drosophila ovaries revealed by GFP protein traps
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DOI：
10.1016/j.ydbio.2007.11.029
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发表时间：
2008-02-15
影响因子：
2.7
通讯作者：
Cooley, Lynn
中科院分区：
文献类型：
--
作者：
Hudson, Andrew M.;Petrella, Lisa N.;Cooley, Lynn
模型系统中肌肉规格、形成和功能的遗传分析为人类肌肉生理学和疾病提供了有价值的见解。在果蝇中的研究对于发现参与肌肉特化、成肌细胞融合和肌节组织的关键基因特别有用。果蝇雌性生殖系统的肌肉很少受到关注，尽管卵子发生的广泛工作。我们已经使用了新的可用的GFP蛋白陷阱线的卵巢肌肉形态和肌节组织的特点。输卵管周围的肌细胞是多核的，具有高度组织化的肌节，是体肌的典型特征。与此相反，卵巢的两个肌肉层，这是来自性腺中胚层，具有类似于肠内脏肌的网状形态。Fasciclin 3基因中的蛋白陷阱产生了Fas 3：：GFP，其定位在上皮鞘细胞周围的点中，即卵巢管周围的肌肉。令人惊讶的是，上皮鞘细胞每个都含有一个细胞核，表明这些细胞在发育过程中不经历成肌细胞融合。与这一观察结果一致，我们能够使用Flp/FRT系统，以有效地产生遗传镶嵌在上皮鞘，这表明这些细胞提供了一个新的机会，成年横纹肌的克隆分析。(c)2008年爱思唯尔公司All rights reserved.
Genetic analysis of muscle specification, formation and function in model systems has provided valuable insight into human muscle physiology and disease. Studies in Drosophila have been particularly useful for discovering key genes involved in muscle specification, myoblast fusion, and sarcomere organization. The muscles of the Drosophila female reproductive system have received little attention despite extensive work on oogenesis. We have used newly available GFP protein trap lines to characterize of ovarian muscle morphology and sarcomere organization. The muscle cells surrounding the oviducts are multinuclear with highly organized sarcomeres typical of somatic muscles. In contrast, the two muscle layers of the ovary, which are derived from gonadal mesoderm, have a mesh-like morphology similar to gut visceral muscle. Protein traps in the Fasciclin 3 gene produced Fas3::GFP that localized in dots around the periphery of epithelial sheath cells, the muscle surrounding ovarioles. Surprisingly, the epithelial sheath cells each contain a single nucleus, indicating these cells do not undergo myoblast fusion during development. Consistent with this observation, we were able to use the Flp/FRT system to efficiently generate genetic mosaics in the epithelial sheath, suggesting these cells provide a new opportunity for clonal analysis of adult striated muscle. (c) 2008 Elsevier Inc. All rights reserved.