Low-input Capture-C: A Chromosome Conformation Capture Assay to Analyze Chromatin Architecture in Small Numbers of Cells.
Low-input Capture-C: A Chromosome Conformation Capture Assay to Analyze Chromatin Architecture in Small Numbers of Cells.
复制标题
低输入 Capture-C：一种染色体构象捕获测定，用于分析少量细胞中的染色质结构。
作者：
A. M. Oudelaar;D. Downes;J. Davies;J. Hughes
染色体构象捕获（3C）技术对于理解基因表达的组织特异性调控至关重要，但目前的方法通常需要大量的细胞。该协议描述了两种新的低输入Capture-C方法，可以从10，000 - 20，000个细胞中生成高质量的3C相互作用谱，具体取决于用于分析的分辨率。
Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis.