Tailor-Made Magnetic Fe3O4@mTiO2 Microspheres with a Tunable Mesoporous Anatase Shell for Highly Selective and Effective Enrichment of Phosphopeptides
Tailor-Made Magnetic Fe3O4@mTiO2 Microspheres with a Tunable Mesoporous Anatase Shell for Highly Selective and Effective Enrichment of Phosphopeptides
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DOI：
10.1021/nn3009646
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发表时间：
2012-04-01
期刊：
影响因子：
17.1
通讯作者：
Wang, Chang-Chun
中科院分区：
文献类型：
--
作者：
Ma, Wan-Fu;Zhang, Ying;Wang, Chang-Chun
从复杂混合物中选择性富集磷蛋白或磷酸肽对于基于 MS 的磷酸蛋白质组学至关重要，但仍然是一个挑战。在本文中，我们描述了一种前所未有的合成磁性介孔Fe3O4@mTiO(2)微球的方法，该微球具有明确的核/壳结构、纯净且高度结晶的TiO2层、高比表面积(167.1 m(2)/g)、大孔体积(0.45 cm(3)/g)、适当且可调的孔径(8.6-16.4 nm)和高磁化率。我们研究了 Fe3O4@mTiO(2) 微球在磷酸肽选择性富集研究中的适用性。实验结果表明，Fe3O4@mTiO(2)即使在极低的磷酸肽/非磷酸肽摩尔比（1:1000）下也对磷酸肽具有显着的选择性、富集容量大（高达225 mg/g，是Fe3O4@TiO2微球的10倍以上）、极高的灵敏度（检测限在fmol水平）、优异的速度（可以在更短的时间内完成富集）。小于5分钟），磷酸肽回收率高（高达93%）。此外，高磁化率允许通过磁分离方便地分离目标肽。这些突出的特性使Fe3O4@mTiO(2)复合微球在磷酸肽的质谱分析中具有很大的优势。
Selective enrichment of phosphoproteins or phosphopeptides from complex mixtures is essential for MS-based phosphoproteomics, but still remains a challenge. In this article, we described an unprecedented approach to synthesize magnetic mesoporous Fe3O4@mTiO(2) microspheres with a well-defined core/shell structure, a pure and highly crystalline TiO2 layer, high specific surface area (167.1 m(2)/g), large pore volume (0.45 cm(3)/g), appropriate and tunable pore size (8.6-16.4 nm), and high magnetic susceptibility. We investigated the applicability of Fe3O4@mTiO(2) microspheres in a study of the selective enrichment of phosphopeptides. The experiment results demonstrated that the Fe3O4@mTiO(2) possessed remarkable selectivity for phosphopeptides even at a very low molar ratio of phosphopeptides/non-phosphopeptides (1:1000), large enrichment capacity (as high as 225 mg/g, over 10 times as that of the Fe3O4@TiO2 microspheres), extreme sensitivity (the detection limit was at the fmol level), excellent speed (the enrichment can be completed in less than 5 min), and high recovery of phosphopeptides (as high as 93%). In addition, the high magnetic susceptibility allowed convenient separation of the target peptides by magnetic separation. These outstanding features give the Fe3O4@mTiO(2) composite microspheres high benefit for mass spectrometric analysis of phosphopeptides.