Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors.
Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors.
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DOI：
10.7554/elife.27454
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发表时间：
2018-02-09
期刊：
影响因子：
7.7
通讯作者：
Shraiman BI
中科院分区：
文献类型：
--
作者：
Streichan SJ;Lefebvre MF;Noll N;Wieschaus EF;Shraiman BI
在胚胎发育过程中，组织层经历形态发生流的重排和折叠成特定的形状。虽然发育生物学已经确定了关键基因和局部细胞过程，但器官规模的组织重塑的全球协调仍不清楚。在这里，我们将果蝇胚胎的光片显微镜与定量分析和物理模型相结合，以将细胞流动与原肠形成过程中的力产生模式联系起来。我们发现，使用一个简单、有效的组织粘性模型，可以根据测量的中尺度肌球蛋白密度和各向异性来预测复杂的时空流动模式，在一个依赖于时间和两个恒定参数的情况下，可以获得接近90%的准确率。我们的分析揭示了a)胚胎尺度上肌球蛋白分布的空间调制以及b)由于细胞的机械相互作用其影响的非局部性的重要性，证明了在形态发生流研究中需要全球视角。
During embryogenesis tissue layers undergo morphogenetic flow rearranging and folding into specific shapes. While developmental biology has identified key genes and local cellular processes, global coordination of tissue remodeling at the organ scale remains unclear. Here, we combine in toto light-sheet microscopy of the Drosophila embryo with quantitative analysis and physical modeling to relate cellular flow with the patterns of force generation during the gastrulation process. We find that the complex spatio-temporal flow pattern can be predicted from the measured meso-scale myosin density and anisotropy using a simple, effective viscous model of the tissue, achieving close to 90% accuracy with one time dependent and two constant parameters. Our analysis uncovers the importance of a) spatial modulation of myosin distribution on the scale of the embryo and b) the non-locality of its effect due to mechanical interaction of cells, demonstrating the need for the global perspective in the study of morphogenetic flow.