The Effects of Rooibos (Aspalathus linearis), Green Tea (Camellia sinensis) and Commercial Rooibos and Green Tea Supplements on Epididymal Sperm in Oxidative Stress-induced Rats
The Effects of Rooibos (Aspalathus linearis), Green Tea (Camellia sinensis) and Commercial Rooibos and Green Tea Supplements on Epididymal Sperm in Oxidative Stress-induced Rats
复制标题
DOI：
10.1002/ptr.3717
复制
发表时间：
2012-08-01
影响因子：
7.2
通讯作者：
Brooks, Nicole
中科院分区：
文献类型：
--
作者：
Awoniyi, Dolapo Olaitan;Aboua, Yapo Guillaume;Brooks, Nicole
活性氧参与哺乳动物精子的多种生理功能。属于酶和非酶基团的许多内源性抗氧化剂可以去除过量的ROS并防止氧化应激（OS）。本研究比较了路易波士，中国绿色茶和商业路易波士和绿色茶补充剂在大鼠精子中对OS的调节。雄性Wistar大鼠（n = 10），60)补充以发酵的路易波士，绿色路易波士，中国绿色茶，路易波士补充剂，绿色茶补充剂或水为10？周，而OS是在过去的2？周与其他组相比，食用发酵路易波士和绿色路易波士的大鼠的精子计数和活力显着较高。过氧化氢酶活性显着较高的大鼠消耗发酵路易波士，绿色路易波士和路易波士和绿色茶补充剂的精子。超氧化物歧化酶浓度的大鼠补充发酵路易波士，绿色路易波士和绿色茶较高。食用发酵路易波士和绿色路易波士的大鼠精子谷胱甘肽水平也明显较高。与对照组相比，发酵路易波士和绿色路易波士具有降低ROS和脂质过氧化水平的趋势。总之，这两种路易波士提取物都可以通过增加抗氧化防御机制来提供针对诱导的氧化损伤的保护措施，从而改善精子质量和功能。版权所有（c）2012约翰威利父子有限公司
Reactive oxygen species (ROS) are involved in many physiological functions of mammalian sperm. Numerous endogenous antioxidants belonging to both enzymatic and non-enzymatic groups can remove excess ROS and prevent oxidative stress (OS). This study compares the modulation of OS by rooibos, Chinese green tea and commercial rooibos and green tea supplements in rat sperm. Male Wistar rats (n?=?60) were supplemented with fermented rooibos, green rooibos, Chinese green tea, rooibos supplement, green tea supplement or water for 10?weeks while OS was induced during the last 2?weeks. Sperm count and motility were significantly higher for rats consuming fermented rooibos and green rooibos when compared with the other groups. Catalase activity was significantly higher in the sperm of rats consuming fermented rooibos, green rooibos and both the rooibos and green tea supplements. Superoxide dismutase concentration in the sperm of rats supplemented with fermented rooibos, green rooibos and green tea was higher. Sperm glutathione levels of rats consuming the fermented and green rooibos were also significantly higher. Rooibos fermented and green rooibos showed a tendency to lower the levels of ROS and lipid peroxidation when compared with the control group. In conclusion, both rooibos extracts could offer a measure of protection against induced oxidative damage by increasing the antioxidant defence mechanisms and thereby improving the sperm quality and function. Copyright (c) 2012 John Wiley & Sons, Ltd.