Regulation of the chitobiose-phosphotransferase system in Vibrio cholerae
Regulation of the chitobiose-phosphotransferase system in Vibrio cholerae
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DOI：
10.1007/s00203-006-0207-4
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发表时间：
2007-06-01
影响因子：
2.8
通讯作者：
Reidl, Joachim
中科院分区：
文献类型：
--
作者：
Berg, Thorsten;Schild, Stefan;Reidl, Joachim
霍乱弧菌拥有磷酸转移酶系统（PTS），使生物体能够利用壳寡糖，例如壳寡糖。源自脱乙酰甲壳素。如最近所示，该利用系统由 ORF VC1281-1283 编码（Meibom 等人，Proc Natl Acad Sci USA，101：2524-2529，2004）。通过使用转录报告基因融合技术，我们确定了系统的调节因子并表征了基因表达。此外，我们发现该 PTS 系统的基因表达受到分解代谢调节以及 Mlc 同源物 (VC2007) 的影响，后者在大肠杆菌中是糖代谢的全局调节因子。
Vibrio cholerae harbours a phosphotransferase system (PTS) enabling the organism to utilise chitosan oligosaccharide, e.g. derived from deacetylated chitin. As shown recently, this utilization system is encoded by the ORFs VC1281-1283 (Meibom et al. in Proc Natl Acad Sci USA, 101:2524-2529, 2004). By using a transcriptional reporter fusion technique, we identified the regulator of the system and characterised gene expression. Furthermore, we found that gene expression of this PTS system is influenced by catabolite regulation and also by an Mlc homologue (VC2007), which in E. coli is a global regulator of sugar metabolism.