Determination of endocrine-disrupting potencies of agricultural soils in China via a battery of steroid receptor bioassays.
Determination of endocrine-disrupting potencies of agricultural soils in China via a battery of steroid receptor bioassays.
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DOI：
10.1016/j.envpol.2017.12.004
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发表时间：
2018-03
影响因子：
8.9
通讯作者：
Jianyun Zhang;Rui Liu;Lili Niu;Siyu Zhu;Quan Zhang;Mei-rong Zhao;Weiping Liu;Jing Liu
中科院分区：
文献类型：
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作者：
Jianyun Zhang;Rui Liu;Lili Niu;Siyu Zhu;Quan Zhang;Mei-rong Zhao;Weiping Liu;Jing Liu
农业土壤的农药污染，如有机氯农药（OCPs），可以是一个重要的问题，因为这些化合物的高检出率在我们以前的研究报告。然而，更多的不确定性污染物的种类，数量和浓度仍然存在于土壤样品中。本研究采用高灵敏度的雌激素受体（ER）、雄激素受体（AR）、孕激素受体（PR）、糖皮质激素受体（GR）和盐皮质激素受体（MR）激动剂/拮抗剂报告基因检测技术，测定了中国大陆农业土壤中已知和未知污染物的复杂混合物的总激素活性。在123个土壤样品中，雌激素活性和抗孕激素活性的检出率很高，分别达到79%和73%。超过一半的土壤样品对AR和GR表现出明显的拮抗作用。约三分之一的测试样品表现出雄激素，孕激素和糖皮质激素的影响。共有72%和78%的土壤提取物具有盐皮质激素样和抗盐皮质激素活性，分别。雌激素活性与DDT、硫丹、氯丹、七氯和异丙肾上腺素的浓度呈显著正相关，而与其它受体的浓度无显著相关性。作为一种快速、简便的预警方法，通过生物测定法测定污染土壤的内分泌干扰能力有助于识别和确定需要进一步关注的生态风险评估场所。
Pollution of agricultural soils by pesticides, such as organochlorine pesticides (OCPs), can be a significant issue since high detection rates of these compounds were reported in our previous studies. However, more uncertain kinds, quantities and density of pollutants remained in soil samples were unidentified. In this study, the total hormonal activities of complex mixtures of both known and unknown contaminants in agricultural soils in mainland China were measured by applying highly sensitive reporter gene assays for detecting agonists/antagonists for estrogen receptor (ER), androgen receptor (AR), progesterone receptor (PR), glucocorticoid receptor (GR) and mineralocorticoid receptor (MR). High detection rates of estrogenic activities and anti-progestogenic activities were observed among the 123 soil samples, reaching 79% and 73%, respectively. More than half of the soil samples showed obvious antagonistic effects against AR and GR. Approximately a third of tested samples exhibited androgenic, progestogenic and glucocorticoidic effects. A total of 72% and 78% soil extracts had mineralocorticoid-like and anti-mineralocorticoid activities, respectively. Significant positive correlations were observed between estrogenic activity and the concentrations of Σdichlorodiphenyltrichloroethanes (DDTs), Σendosulfans, Σchlordanes, heptachlor and Σdrins, respectively, but not other receptors. As a rapid and convenient pre-caution method, determination of endocrine-disrupting potencies of contaminated soils via bioassay could help to identify and define sites that required further attention for ecological risk assessments.