Evolutionarily Dynamic, but Robust, Targeting of Resistance Genes by the miR482/2118 Gene Family in the Solanaceae.
Evolutionarily Dynamic, but Robust, Targeting of Resistance Genes by the miR482/2118 Gene Family in the Solanaceae.
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DOI：
10.1093/gbe/evv225
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发表时间：
2015-11-19
影响因子：
3.3
通讯作者：
Rose LE
中科院分区：
文献类型：
--
作者：
de Vries S;Kloesges T;Rose LE
植物全天候暴露在病原体之下。植物对病原菌检测到的一种常见的抗性反应是局部的细胞死亡。鉴于这种反应的不可逆性，存在多层负调控，以防止抗性基因的过早或错误表达。一层负调控是由最近发现的microRNA(MiRNA)基因家族miR482/2118提供的。这个家族的目标是植物中抗性基因的转录本。我们研究了该miRNA基因家族在茄科植物中的进化历史和特异性。这个植物家族包括许多重要的作物物种，提供了一套明确的抗性基因库。在茄科14种植物中，我们鉴定了8个不同的miR482/2118基因家族成员。我们的研究表明，miRNA的类型和数量在野生番茄群体中是保守的，在较小程度上，在整个茄科植物中也是如此。8个同源miRNA基因簇在不同的进化约束下进化，允许miRNAs的个体亚功能化。尽管每个miRNA的预测靶向行为不同，但miRNA-R基因网络由于其高度的互连和冗余的靶向而是稳健的。我们的数据表明，miR482/2118基因家族是R基因序列多样性的进化缓冲区。
Plants are exposed to pathogens around the clock. A common resistance response in plants upon pathogen detection is localized cell death. Given the irreversible nature of this response, multiple layers of negative regulation are present to prevent the untimely or misexpression of resistance genes. One layer of negative regulation is provided by a recently discovered microRNA (miRNA) gene family, miR482/2118. This family targets the transcripts of resistance genes in plants. We investigated the evolutionary history and specificity of this miRNA gene family within the Solanaceae. This plant family includes many important crop species, providing a set of well-defined resistance gene repertoires. Across 14 species from the Solanaceae, we identified eight distinct miR482/2118 gene family members. Our studies show conservation of miRNA type and number in the group of wild tomatoes and, to a lesser extent, throughout the Solanaceae. The eight orthologous miRNA gene clusters evolved under different evolutionary constraints, allowing for individual subfunctionalization of the miRNAs. Despite differences in the predicted targeting behavior of each miRNA, the miRNA–R-gene network is robust due to its high degree of interconnectivity and redundant targeting. Our data suggest that the miR482/2118 gene family acts as an evolutionary buffer for R-gene sequence diversity.