Engineering temperature-sensitive hydrogel nanoparticles entrapping hemoglobin as a novel type of oxygen carrier
Engineering temperature-sensitive hydrogel nanoparticles entrapping hemoglobin as a novel type of oxygen carrier
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DOI：
10.1021/bm050144b
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发表时间：
2005-07-01
影响因子：
6.2
通讯作者：
Palmer, AF
中科院分区：
文献类型：
--
作者：
Patton, JN;Palmer, AF
响应于温度变化同时保持胶体稳定性的温度敏感性氧载体将有益于以由于体温降低而导致的组织缺氧为特征的生理条件。这些病症通常伴随着血流减少和血管收缩。当体温降低时，温度敏感性氧载体应该理想地具有增加的氧亲和力，以选择性地靶向由于温度下降而缺氧的组织。该研究扩展了先前的工作，其引入了基于水凝胶的氧载体作为一类新的氧载体，其可以通过光引发的自由基聚合在脂质体反应器内合成[Patton，J.N.; Palmer，A. F. Biomacromolecules 2005，6，414-24]。除了封装牛血红蛋白的聚（N-异丙基丙烯酰胺）水凝胶纳米颗粒响应于温度的生理变化而膨胀和收缩的能力之外，还检查了温度变化对ζ电位、氧亲和力和协同性的影响。高铁血红蛋白水平和血红蛋白的水凝胶基氧载体的封装效率也被提出。观察到纳米级水凝胶颗粒随着温度从40 ° C降低到29 ° C而溶胀，这表明水凝胶基质膨胀并且对氧转运的阻力降低。
Temperature-sensitive oxygen carriers that are responsive to changes in temperature while maintaining colloidal stability would benefit physiological conditions characterized by tissue hypoxia due to decreased body temperature. These conditions are often accompanied with reduced blood flow and vasoconstriction. Temperature-sensitive oxygen carriers should ideally possess increased oxygen affinity when the body temperature is reduced, to selectively target tissues that are hypoxic as a result of temperature drops. This study expands on previous work, which introduced hydrogel based oxygen carriers as a new class of oxygen carrier that can be synthesized within liposomal reactors via photoinitiated free radical polymerization [Patton, J. N.; Palmer, A. F. Biomacromolecules 2005, 6, 414-24]. In addition to the ability of poly(N-isopropylacrylarnide) hydrogel nanoparticles encapsulating bovine hemoglobin to swell and shrink in response to physiological changes in temperature, the effect of temperature changes on zeta potential, oxygen affinity, and cooperativity are also examined. The methemoglobin level and hemoglobin encapsulation efficiency of hydrogel-based oxygen carriers are also presented. It was observed that nanoscale hydrogel particles swelled as the temperature decreased from 40 to 29 degrees C, which suggests expansion of the hydrogel matrix and reduced resistance to oxygen transport.