Live Helicobacter pylori in the root canal of endodontic-infected deciduous teeth
Live Helicobacter pylori in the root canal of endodontic-infected deciduous teeth
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DOI：
10.1007/s00535-012-0618-8
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发表时间：
2012-08-01
影响因子：
6.3
通讯作者：
Backert, Steffen
中科院分区：
文献类型：
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作者：
Hirsch, Christian;Tegtmeyer, Nicole;Backert, Steffen
许多基于聚合酶链式反应(PCR)的研究表明，幽门螺杆菌DNA在口腔中普遍存在，但有关活菌分离的报道极其罕见。因此，幽门螺杆菌是否真的能在口腔环境中存活尚不清楚。在这里，我们使用电子显微镜、选择性生长技术、尿素酶分析、16S rRNA聚合酶链式反应(16S RRNA PCR)和免疫印迹法研究了幽门螺杆菌在3名儿童根管感染乳牙的10个根管和相应的菌斑样本中是否存在活的幽门螺杆菌。尽管在一些菌斑和根管样本中通过聚合酶链式反应证实了幽门螺杆菌的DNA，但细菌菌落只能从两个根管中生长，而不能从菌斑中生长。这些菌落通过显微镜、遗传和生化方法被明确地鉴定为幽门螺杆菌。我们的发现表明，根管感染的牙齿的根管可能是活的幽门螺杆菌的储存库，可能是潜在的传播源。
Many polymerase chain reaction (PCR)-based studies have shown that Helicobacter pylori DNA is prevalent in the oral cavity, but reports on the isolation of live bacteria are extremely rare. Thus, it is still unclear whether H. pylori can indeed survive in the oral environment.Here we used electron microscopy, selective growth techniques, urease assays, 16S rRNA PCR, and western blotting to investigate the possible presence of live H. pylori in 10 root canal and corresponding plaque samples of endodontic-infected deciduous teeth in three children.Although H. pylori DNA was verifiable by PCR in several plaque and root canal samples, bacterial colonies could only be grown from two root canals, but not from plaque. These colonies were unequivocally identified as H. pylori by microscopic, genetic, and biochemical approaches.Our findings show that root canals of endodontic-infected teeth may be a reservoir for live H. pylori that could serve as a potential source for transmission.