Purification and characterization of bacteriocin F1, a novel bacteriocin produced by Lactobacillus paracasei subsp tolerans FX-6 from Tibetan kefir, a traditional fermented milk from Tibet, China
Purification and characterization of bacteriocin F1, a novel bacteriocin produced by Lactobacillus paracasei subsp tolerans FX-6 from Tibetan kefir, a traditional fermented milk from Tibet, China
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细菌素 F1 的纯化和表征，细菌素 F1 是一种由副干酪乳杆菌亚种产生的新型细菌素。
DOI：
10.1016/j.foodcont.2014.01.041
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发表时间：
2014-08-01
期刊：
影响因子：
6
通讯作者：
Cao, Yong
中科院分区：
文献类型：
--
作者：
Miao, Jianyin;Guo, Haoxian;Cao, Yong
使用琼脂井扩散测定从西藏开菲尔中分离出一种产生细菌素的乳酸菌 FX-6。菌株FX-6被鉴定为副干酪乳杆菌亚种。耐受性基于 16S rDNA 序列分析。该细菌素被命名为细菌素F1，通过三步纯化程序进行纯化。 MALDI-TOF MS分析得出细菌素F1的分子量为2113.842 Da。它还被发现阻断了 N 末端，阻碍了 Edman 降解分析。细菌素Fl表现出广泛的抗菌活性、较强的热稳定性（121℃下20分钟，或100℃下60分钟）和pH稳定性（pH 3.0-9.0）。经胃蛋白酶和胰蛋白酶处理后，抗菌活性部分降低。细菌素 Fl 是第一个报道的由副干酪乳杆菌亚种产生的细菌素。耐受菌不仅能抑制真菌，还能抑制细菌。细菌素Fl的表征表明它是一种新型细菌素，在食品工业中具有潜在的研究和应用价值。 (C) 2014 Elsevier Ltd. 保留所有权利。
A bacteriocin-producing lactic acid bacteria, FX-6, was isolated from Tibetan kefir using the agar well diffusion assay. Strain FX-6 was identified as Lactobacillus paracasei subsp. tolerans on the basis of 16S rDNA sequence analyses. This bacteriocin, which was designated bacteriocin Fl, was purified by a threestep purification procedure. The molecular mass of bacteriocin Fl was 2113.842 Da by MALDI-TOF MS analyses. It was also discovered to have blocked N-termini, hampering analysis by Edman degradation. Bacteriocin Fl exhibited a wide range of antimicrobial activity, strong heat stability (20minat 121 C, or 60 min at 100 degrees C) and pH stability (pH 3.0-9.0). Following treatment by pepsin and trypsin, the antibacterial activity was partly reduced. Bacteriocin Fl is the first reported bacteriocin produed by L paracasei subsp. tolerans which can not only inhibit fungi but also bacteria. The characterization of bacteriocin Fl suggested that it was a novel bacteriocin with potential research and application value in food industry. (C) 2014 Elsevier Ltd. All rights reserved.