A new model for embryo implantation: coculture of blastocysts and Ishikawa cells
A new model for embryo implantation: coculture of blastocysts and Ishikawa cells
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DOI：
10.3109/09513590.2011.631623
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发表时间：
2012-03
影响因子：
2
通讯作者：
Dan Zhang;P. Lv;Runju Zhang;Qiong Luo;Guolian Ding;Li-Qiang Yin;Jingyi Li;Gu-feng Xu;F. Qu;Jianzhong Sheng;He-Feng Huang
中科院分区：
文献类型：
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作者：
Dan Zhang;P. Lv;Runju Zhang;Qiong Luo;Guolian Ding;Li-Qiang Yin;Jingyi Li;Gu-feng Xu;F. Qu;Jianzhong Sheng;He-Feng Huang
目的：探索和建立一种能反映胚胎贴壁和侵袭主要过程的体外着床模型。研究设计：人类胚胎植入研究的局限性之一是缺乏一个忠实地复制人类胚胎-子宫相互作用的体外模型。在本研究中，我们研究了小鼠囊胚在人子宫内膜细胞石川细胞（IK）中的附着和侵袭能力，以阐明这种新的模型是否适合研究胚胎植入。我们使用IK并将其与囊胚接触，以使用专门设计的培养基启动共培养实验。培养液由Ham F-12/Dulbecco改良Eagle培养基（1：1）、30%胎牛血清、63.5 nmol/L孕酮、7.14 nmol/L雌二醇-17 β、100 mg/ml胰岛素和20 ng/ml表皮生长因子组成。培养24 h清楚地表明，胚胎能够附着到IK上，并显示出部分侵袭。结果：囊胚与IK共培养48 h后，胚胎贴壁并部分侵入IK。结论：该模型能够显示胚胎在子宫内膜细胞中的附着和侵入过程，并有望用于与人类子宫内膜早期胚胎植入相关的研究。
Objective: To explore and develop a new in vitro implantation model that reflects the main process of embryo attachment and invasion. Study design: One of the limitations in human embryo implantation research is lack of an available in vitro model that faithfully replicates human embryo–uterine interactions. In the present study, we examined the attachment and invasiveness of blastocysts from mice in Ishikawa cell (IK), a human endometrial cell, to clarify whether this new model is suitable to study implantation of embryos. We used IK and placed it in contact with blastocysts to initiate coculture experiments using a specifically designed medium. The culture medium was composed of Ham F-12/Dulbecco’s modified Eagle medium (1:1), 30% fetal calf serum, 63.5 nmol/L progesterone, 7.14 nmol/L estradiol-17β, 100 mg/ml of insulin, and 20 ng/ml epidermal growth factor. The culture for 24 h clearly demonstrated that embryos were capable of attachment to the IK and displayed partial invasion. Results: Our results showed that embryos attached to the IK and displayed partial invasion after coculture of blastocysts with IK for 48 h. Conclusions: The model is capable of demonstrating the procedure of attachment and invasion of embryo into the endometrial cells and has promises to be used in studies related to early embryo implantation in human endometrium.