Reactive radical-driven bacterial inactivation by hydrogen-peroxide-enhanced plasma-activated-water
Reactive radical-driven bacterial inactivation by hydrogen-peroxide-enhanced plasma-activated-water
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DOI：
10.1140/epjst/e2016-60330-y
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发表时间：
2017-08-01
影响因子：
2.8
通讯作者：
Fang, Jing
中科院分区：
文献类型：
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作者：
Wu, Songjie;Zhang, Qian;Fang, Jing
本研究调查了等离子体活化水 (PAW) 和过氧化氢 (H2O2)、PAW/HP 在灭菌中的综合作用。为了评估PAW/HP的协同效应，选择金黄色葡萄球菌作为测试微生物来确定灭活效果。此外，还检查了不同条件下细菌悬浮液释放的 DNA/RNA 和蛋白质，以确认膜的完整性。此外，我们的研究还测量了金黄色葡萄球菌的细胞内 pH (pH(i))。使用电子自旋共振光谱（ESR）来识别自由基的存在。最后，测量氧化还原电位（ORP）、电导率和pH值。我们的结果表明，PAW/HP 的灭活效率远高于 PAW，而增加 H2O2 浓度会导致更高的灭活潜力。更重要的是，与PAW相比，PAW/HP中更强的ESR信号强度和更高的ORP表明PAW/HP协同效应的灭活机制是：PAW与H2O2结合产生更多的活性氧（ROS）和活性氮（RNS），特别是OH和NO自由基，导致更多细菌死亡。
The combined effects of plasma activated water (PAW) and hydrogen peroxide (H2O2), PAW/HP, in sterilization were investigated in this study. To assess the synergistic effects of PAW/HP, S. aureus was selected as the test microorganism to determine the inactivation efficacy. Also, the DNA/RNA and proteins released by the bacterial suspensions under different conditions were examined to confirm membrane integrity. Additionally, the intracellular pH (pH(i)) of S. aureus was measured in our study. Electron spin resonance spectroscopy (ESR) was employed to identify the presence of radicals. Finally, the oxidation reduction potential (ORP), conductivity and pH were measured. Our results revealed that the inactivation efficacy of PAW/HP is much greater than that of PAW, while increased H2O2 concentration result in higher inactivation potential. More importantly, as compared with PAW, the much stronger intensity ESR signals and higher ORP in PAW/HP suggests that the inactivation mechanism of the synergistic effects of PAW/HP: more reactive oxygen species (ROS) and reactive nitrogen species (RNS), especially OH and NO radicals, are generated in PAW combined with H2O2 resulting in more deaths of the bacteria.