Chemical synthesis of a polypeptide predicted from nucleotide sequence allows detection of a new retroviral gene product
Chemical synthesis of a polypeptide predicted from nucleotide sequence allows detection of a new retroviral gene product
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从核苷酸序列预测的多肽的化学合成允许检测新的逆转录病毒基因产物
作者：
J. Sutcliffe;T. Shinnick;N. Green;Fuan Liu;H. Niman;R. Lerner
我们先前测定了莫洛尼白血病病毒3′端的核苷酸序列，并发现了一种未知蛋白R.我们现在表明该区域确实编码蛋白质。用化学方法合成了R的十五肽，并制备了抗该肽的抗体，该合成肽的抗血清识别感染细胞中的R蛋白和env前体多聚蛋白。这里提出的策略应该提供一个通用的方法来访问预测的蛋白质的核苷酸序列。
We previously determined the nucleotide sequence of the 3′ end of Moloney leukaemia virus and discovered the potential coding region for an unknown protein, R. We now show that this region does encode a protein. A pentadecapeptide of R was chemically synthesized and antibodies raised against it. Antisera to the synthetic peptide recognize the R protein and the env precursor polyprotein in infected cells. The strategy presented here should provide a general method for accessing proteins predicted by nucleotide sequences.
使用单克隆抗体对 Rauscher 病毒 gp70 进行分子解剖：相关包膜基因重组体的获得序列的定位。
DOI：
10.1073/pnas.77.8.4524
发表时间：
1980
影响因子：
11.1
作者：
Niman,HL;Elder,JH
通讯作者：
Elder,JH