Proliferation rate of stem cells derived from human dental pulp and identification of differentially expressed genes
Proliferation rate of stem cells derived from human dental pulp and identification of differentially expressed genes
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DOI：
10.1002/cbin.10229
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发表时间：
2014-05-01
影响因子：
3.9
通讯作者：
Mokhtar, Khairani Idah
中科院分区：
文献类型：
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作者：
Abdullah, Muhammad Fawwaz;Abdullah, Siti Fadilah;Mokhtar, Khairani Idah
培养来自人脱落乳牙的干细胞（SHED）和从人拔出的牙齿的牙髓获得的牙髓干细胞（DPSC）并进行表征，以确认这些是间充质干细胞。使用AlamarBlue（R）细胞测定评估增殖率。采用GeneFishing（TM）技术鉴定SHED和DPSC中差异表达的基因。SHED的增殖率显著高于DPSCs（P < 0.05），但其增殖率和群体倍增时间（0.01429，60.57 h）均低于DPSCs（0.00286，472.43 h）。SHED中有两条高表达带，DPSC中有三条高表达带。测序分析显示，这些是TIMP金属肽酶抑制剂1（TIMP 1），核糖体蛋白s8（RPS 8）在SHED和胶原蛋白，I型，α 1，（COL 1A 1），卵泡抑素样1（FSTL 1），凝集素，半乳糖苷结合，可溶性，1，（LGALS 1）在DPSC。TIMP 1参与细胞外基质的降解、细胞增殖和抗凋亡功能，RPS 8作为限速因子参与翻译调节; COL 1A 1参与组织的抵抗力和弹性; FSTL 1是与类风湿性关节炎相关的自身抗原; LGALS 1参与细胞生长、分化、粘附、RNA加工、凋亡和恶性转化。这一点，沿着进一步的蛋白质表达分析，在组织工程和再生医学中有希望。
Stem cells from human exfoliated deciduous teeth (SHED) and dental pulp stem cells (DPSCs) obtained from the dental pulp of human extracted tooth were cultured and characterized to confirm that these were mesenchymal stem cells. The proliferation rate was assessed using AlamarBlue (R) cell assay. The differentially expressed genes in SHED and DPSCs were identified using the GeneFishing (TM) technique. The proliferation rate of SHED (P < 0.05) was significantly higher than DPSCs while SHED had a lower multiplication rate and shorter population doubling time (0.01429, 60.57 h) than DPSCs (0.00286, 472.43 h). Two bands were highly expressed in SHED and three bands in DPSCs. Sequencing analysis showed these to be TIMP metallopeptidase inhibitor 1 (TIMP1), and ribosomal protein s8, (RPS8) in SHED and collagen, type I, alpha 1, (COL1A1), follistatin-like 1 (FSTL1), lectin, galactoside-binding, soluble, 1, (LGALS1) in DPSCs. TIMP1 is involved in degradation of the extracellular matrix, cell proliferation and anti-apoptotic function and RPS8 is involved as a rate-limiting factor in translational regulation; COL1A1 is involved in the resistance and elasticity of the tissues; FSTL1 is an autoantigen associated with rheumatoid arthritis; LGALS1 is involved in cell growth, differentiation, adhesion, RNA processing, apoptosis and malignant transformation. This, along with further protein expression analysis, holds promise in tissue engineering and regenerative medicine.