An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression.
An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression.
复制标题
与人类 21 号染色体相关的活性允许腺病毒 E1B-55K 和 E4orf6 蛋白在核内共定位，并促进病毒晚期基因表达。
DOI：
10.1128/jvi.77.14.8087-8098.2003
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发表时间：
2003
影响因子：
5.4
通讯作者：
Ornelles,DavidA
中科院分区：
文献类型：
--
作者：
Chastain-Moore,AmyM;Roberts,Terry;Trott,DeborahA;Newbold,RobertF;Ornelles,DavidA
腺病毒E1 B-55 K和E4 orf 6蛋白在病毒感染期间合作，同时执行有助于生产性感染的几项任务，包括晚期病毒mRNA的选择性核质转运。先前的研究表明，E4 orf 6蛋白保留了人类和猴子细胞核中的E1 B-55 K蛋白，但不在啮齿动物细胞核中，这表明灵长类动物特异性细胞因子有助于E4 orf 6介导的E1 B-55 K蛋白保留在细胞核中。为了鉴定这些提议的灵长类动物特异性细胞因子，在一组稳定的人-啮齿动物单染色体体细胞杂交体中研究了E1 B-55 K和E4 orf 6蛋白的相互作用。对这组细胞系的分析表明，存在与人21号染色体相关的活性，该活性允许E1 B-55 K和E4 orf 6蛋白在啮齿动物细胞的细胞核中共定位。携带人类21号染色体部分的其他杂交细胞用于将这种活性映射到染色体的10兆碱基对片段，从21q22.12延伸到q末端附近的区域。引人注目的是，该区域还促进了腺病毒晚期基因在啮齿动物细胞背景中的表达，而对早期病毒基因的表达几乎没有影响。
The adenovirus E1B-55K and E4orf6 proteins cooperate during virus infection while performing several tasks that contribute to a productive infection, including the selective nucleocytoplasmic transport of late viral mRNA. Previous studies have shown that the E4orf6 protein retains the E1B-55K protein in the nucleus of human and monkey cells, but not in those of rodents, suggesting that primate-specific cellular factors contribute to the E4orf6-mediated retention of the E1B-55K protein in the nucleus. In an effort to identify these proposed primate-specific cellular factors, the interaction of the E1B-55K and E4orf6 proteins was studied in a panel of stable human-rodent monochromosomal somatic cell hybrids. Analysis of this panel of cell lines has demonstrated the existence of an activity associated with human chromosome 21 that permits the E1B-55K and E4orf6 proteins to colocalize in the nucleus of a rodent cell. Additional hybrid cells bearing portions of human chromosome 21 were used to map this activity to a 10-megabase-pair segment of the chromosome, extending from 21q22.12 to a region near the q terminus. Strikingly, this region also facilitates the expression of adenovirus late genes in a rodent cell background while having little impact on the expression of early viral genes.