Wireless logging of extracellular neuronal activity in the telencephalon of free-swimming salmonids
Wireless logging of extracellular neuronal activity in the telencephalon of free-swimming salmonids
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DOI：
10.1186/s40317-021-00232-4
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发表时间：
2021-02-12
影响因子：
2.7
通讯作者：
Makiguchi, Yuya
中科院分区：
文献类型：
--
作者：
Takahashi, Susumu;Hombe, Takumi;Makiguchi, Yuya
背景鲑鱼回到他们出生的河流中，这种现象被称为母亲河迁移。人们广泛研究了移徙的基础，特别是关于外部环境线索的行为相关性，如母亲河的气味和地磁罗盘。然而，神经元基础仍然难以捉摸，因为还没有适合监测大型自由游动鱼类大脑神经元活动的生物记录技术。在这项研究中，我们开发了一个无线生物记录系统，以记录细胞外神经元活动在大脑中的自由游泳salmonids.ResultsUsing这个系统，我们记录了多种神经元活动从端脑的鳟鱼游泳在一个矩形的水箱。作为原理的证明，我们检查了细胞外尖峰波形和时序的活动统计。我们发现细胞在响应特定的头部方向时最大限度地放电，类似于啮齿动物大脑中发现的头部方向细胞。我们的研究结果表明，所记录的信号起源于neurons.ConclusionsWe预计，我们的biologging系统将促进更详细的调查到神经的鱼运动的基础，使用内部产生的信息，包括对外部线索的反应。
BackgroundSalmonids return to the river where they were born in a phenomenon known as mother-river migration. The underpinning of migration has been extensively examined, particularly regarding the behavioral correlations of external environmental cues such as the scent of the mother-river and geomagnetic compass. However, neuronal underpinning remains elusive, as there have been no biologging techniques suited to monitor neuronal activity in the brain of large free-swimming fish. In this study, we developed a wireless biologging system to record extracellular neuronal activity in the brains of free-swimming salmonids.ResultsUsing this system, we recorded multiple neuronal activities from the telencephalon of trout swimming in a rectangular water tank. As proof of principle, we examined the activity statistics for extracellular spike waveforms and timing. We found cells firing maximally in response to a specific head direction, similar to the head direction cells found in the rodent brain. The results of our study suggest that the recorded signals originate from neurons.ConclusionsWe anticipate that our biologging system will facilitate a more detailed investigation into the neural underpinning of fish movement using internally generated information, including responses to external cues.