Advances in Applications of Fluorescent Probes Based on Indole Heptamethine in Vivo
Advances in Applications of Fluorescent Probes Based on Indole Heptamethine in Vivo
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DOI：
10.6023/a13070689
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发表时间：
2013
影响因子：
2.5
通讯作者：
Ningning Chu;Chengliang Feng;Min Ji
中科院分区：
文献类型：
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作者：
Ningning Chu;Chengliang Feng;Min Ji
菁染料是一种市售的近红外荧光染料，其光谱范围在近红外区，在此光谱范围内，生物样品的吸收或荧光强度都很小，因此应用菁染料对活体进行成像可以减少背景干扰。吲哚七甲川荧光探针是一类典型的菁染料，由吲哚杂环、七甲川和N-取代侧链组成。由于它们具有良好的溶解性、可调的最大吸收波长、较大的摩尔消光系数等优良的光物理性质，被广泛应用于靶向肿瘤治疗、蛋白质标记、金属阳离子检测等生物学方面。基于吲哚七甲川的结构，在母体化合物上引入反应基团或改变其结构，使荧光探针具有标记蛋白质、肿瘤、检测细胞内金属离子等不同功能，已成为荧光成像生物学研究领域的热点。本文综述了吲哚七甲川类荧光探针在金属离子、pH变化、分子检测以及肿瘤、蛋白质活体成像等方面的应用进展。此外，还阐述了探针在体内（模型）的分布、成像结果和代谢情况。最后讨论了该类荧光探针的生物应用前景及存在的问题。吲哚七甲川;荧光;探针;生物;应用
Cyanine dye is a commercially available near-infrared fluorescent dye, its spectral range is in the near infrared region, in this spectrum, absorption or fluorescence intensity of biological sample is very small, so applied cyanine dye to image living body can reduce background interference. Indole heptamethine fluorescent probes which are representative of cyanine dyes are consisted of indole heterocycle, heptamethine and N-substituted side chains. They are widely used for tar- geted cancer therapy, labling protein, detecting of metal cations and other biological aspects because of their excellent pho- tophysical properties like good solubility, tunable maximum absorption wavelength, large molar extinction coefficient. Based on the structure of indole heptamethine, introducing reactive groups to the parent compounds or changing their structures can make fluorescent probes have different functions like labeling protein and tumour, detecting intracellular metal cations, which has become the hotspot in the field of fluorescence imaging of biological research. Therefore in this paper, the recent progress in the field of applications of indole heptamethine based fluorescent probes for detecting metal cations, changes of pH, mol- ecules and imaging cancer, protein in vivo are reviewed. Besides, the distribution, imaging results and metabolism of probes in vivo (model) have also been elaborated. The biological application trends and problems of this kind of fluorescent probes are also discussed in the end. Keywords indole heptamethine; fluorescent; probe; biological; application