Exploration of induced sputum BIRC3 levels and clinical implications in asthma.
Exploration of induced sputum BIRC3 levels and clinical implications in asthma.
复制标题
哮喘患者诱导痰BIRC 3水平及其临床意义的探讨
DOI：
10.1186/s12890-022-01887-2
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发表时间：
2022-03-14
影响因子：
3.1
通讯作者：
Guo Y
中科院分区：
文献类型：
--
作者：
Du L;Xu C;Zeng Z;Chen F;Tang K;Liang Y;Guo Y
杆状病毒 IAP 重复序列 3 (BIRC3)，编码在哮喘表达谱数据集中上调的 IAP 蛋白家族成员。然而，很少有研究探讨BIRC3在哮喘中的临床意义。验证BIRC3在哮喘诱导痰中的表达及其临床意义。基于GSE76262（118例哮喘病例和21例健康对照）数据集，使用R软件筛选差异表达基因。随后，通过定量实时聚合酶链反应（qRT-PCR）和酶联免疫吸附测定（ELISA）在诱导痰样本中对BIRC3 mRNA和蛋白质进行了临床验证。此外，分析BIRC3表达与哮喘嗜酸性粒细胞/过敏性炎症指标（FeNO、IgE和EOS％）、肺功能（FEV1、FEV1％pred、FVC％pred和FEV1/FVC）和炎症细胞因子（IL-4、IL-5、IL-13、IL-25、IL-10、IL-33和TSLP）之间的相关性。最后，在细胞因子（IL-4或IL-13）刺激的人原代支气管上皮细胞中检测到BIRC3 mRNA。 BIRC3被筛选为GSE76262中的候选基因，该基因在哮喘中高表达。高表达的BIRC3与嗜酸性粒细胞和过敏指标，包括FeNO、血液嗜酸性粒细胞和血清IgE呈正相关。此外，BIRC3蛋白与炎症细胞因子如IL-4、IL-5、IL-13、IL-25、IL-10、IL-33和TSLP呈正相关，而与FEV1、FEV1%pred、FVC%pred和FEV1/FVC呈负相关。此外，细胞因子IL-4或IL-13处理的原代支气管上皮细胞中可以诱导BIRC3的表达。 BIRC3在哮喘诱导痰中显着升高，并与气道嗜酸性粒细胞和外周血过敏性炎症、2型细胞因子和气道阻塞呈正相关。 BIRC3 增加可能通过影响嗜酸性粒细胞和过敏性炎症参与哮喘的发病机制。在线版本包含可在 10.1186/s12890-022-01887-2 获取的补充材料。
Baculoviral IAP repeat-containing 3 (BIRC3) which encodes a member of the IAP family of proteins upregulated in the asthma expression profile dataset. However, there was few research on studying the clinical implication of BIRC3 in asthma. To validate BIRC3 expression and its clinical implications in induced sputum of asthma. Based on the GSE76262 (118 asthma cases and 21 healthy controls) dataset, differentially expressed genes were screened using R software. Subsequently, BIRC3 mRNA and protein were clinically verified in induced sputum samples through quantitative real‐time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). Besides, the correlations between BIRC3 expression and asthmatic eosinophilic/allergic inflammation indicators (FeNO, IgE, and EOS%), pulmonary function (FEV1, FEV1% pred, FVC% pred, and FEV1/FVC), and inflammatory cytokines (IL-4, IL-5, IL-13, IL-25, IL-10, IL-33, and TSLP) were analyzed. Finally, BIRC3 mRNA was detected in human primary bronchial epithelial cells stimulated by cytokines (IL-4 or IL-13). BIRC3 was screened as a candidate gene in the GSE76262, which was highly expressed in asthma. Highly expressed BIRC3 was positively correlated with eosinophilic and allergic indicators, including FeNO, blood eosinophil, and serum IgE. Moreover, BIRC3 protein was positively associated with inflammation cytokines, like IL-4, IL-5, IL-13, IL-25, IL-10, IL-33, and TSLP, while negatively correlated with FEV1, FEV1%pred, FVC% pred, and FEV1/FVC. Furthermore, the expression of BIRC3 could be induced in primary bronchial epithelial cells treated by cytokines IL-4 or IL-13. BIRC3 significantly increased in induced sputum of asthma and positively correlated with airway eosinophilic and peripheral blood allergic inflammation, type 2 cytokines, and airway obstruction. Increased BIRC3 might be involved in the pathogenesis of asthma by affecting the eosinophilic and allergic inflammation. The online version contains supplementary material available at 10.1186/s12890-022-01887-2.