Bacterial imaging with photostable upconversion fluorescent nanoparticles
Bacterial imaging with photostable upconversion fluorescent nanoparticles
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DOI：
10.1016/j.biomaterials.2013.12.060
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发表时间：
2014-03-01
期刊：
影响因子：
14
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
--
作者：
Ong, Li Ching;Ang, Lei Yin;Zhang, Yong
使用下转换荧光团和荧光蛋白进行细菌标记时，经常会遇到自发荧光、光损伤和光漂白。当试图绘制长期细菌传播图时，这些警告代表了严重的限制。上转换纳米粒子 (UCN) 能够将低能量近红外 (NIR) 激发光转换为更高能量的可见光或近红外光，可以解决这些限制。这些颗粒独特的光学特性转化为最小的自发荧光、减少的光损伤、更深的组织渗透和延长的光稳定性等极具吸引力的优势。在这里，我们报告了一种基于 UCN 的细菌标记策略，使用大肠杆菌作为原型细菌。比较分析强调了 UCN 标记细菌优于表达绿色荧光蛋白的细菌的光稳定性。树突状细胞中 UCN 标记细菌的感染研究表明，感染后长达 6 小时 UCN 信号与细菌位置共定位。此外，对相同感染细胞的长期监测证明了利用基于光稳定 UCN 的成像进行细菌贩运的潜力。 (C) 2013 Elsevier Ltd. 保留所有权利。
Autofluorescence, photodamage and photobleaching are often encountered when using downconverting fluorophores and fluorescent proteins for bacteria labeling. These caveats represent a serious limitation when trying to map bacteria dissemination for prolonged periods. Upconversion nanoparticles (UCNs), which are able to convert low energy near-infrared (NIR) excitation light into higher energy visible or NIR light, can address these limitations. These particles' unique optical properties translate into attractive advantages of minimal autofluorescence, reduced photodamage, deeper tissue penetration and prolonged photostability. Here, we report a UCN-based bacteria labeling strategy using Escherichia coli as prototypic bacteria. A comparative analysis highlighted the superior photostability of UCN-labeled bacteria over green fluorescent protein-expressing bacteria. Infection study of UCN-labeled bacteria in dendritic cells indicated co-localization of the UCN signal with bacterial position for up to 6 h post-infection. Furthermore, long-term monitoring of the same infected cells demonstrated the potential to utilize photostable UCN-based imaging for bacterial trafficking purposes. (C) 2013 Elsevier Ltd. All rights reserved.