High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells
High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells
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DOI：
10.1042/0264-6021:3420497
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发表时间：
1999-09-15
影响因子：
4.1
通讯作者：
Estibeiro, P
中科院分区：
文献类型：
--
作者：
Duncan, RR;Don-Wauchope, AC;Estibeiro, P
肾上腺嗜铬细胞常用于胞吐作用的研究。分子机制的表征进展缓慢，因为没有简单、高效的技术可用于在嗜铬细胞中引入和表达异源 cDNA。在这里，我们证明塞姆利基森林病毒（SFV）载体可以在嗜铬细胞中高效表达异源蛋白。
Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.