UV–Vis Spectrophotometric Analysis and Quantification of Glyphosate for an Interdisciplinary Undergraduate Laboratory
UV–Vis Spectrophotometric Analysis and Quantification of Glyphosate for an Interdisciplinary Undergraduate Laboratory
复制标题
跨学科本科实验室草甘膦的紫外-可见分光光度分析和定量
DOI：
--
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
K. Waynant
中科院分区：
文献类型：
--
作者：
Daniel Felton;M. Ederer;T. Steffens;P. Hartzell;K. Waynant
甘氨酰（N-（膦酰基甲基）甘氨酸）是地球上使用最广泛的除草剂。一个简单的测定，以量化草甘膦浓度在环境样品中开发的一部分，跨学科的努力，连接在化学，生物学和微生物学的介绍性实验室课程。在这个3小时的实验室实验中，学生们使用紫外-可见光谱法对草甘膦处理过的土壤样品中制备的未知物和上清液中的草甘膦进行定量。回归分析表明，该测定是线性高达20.0 ppm，使其特别适用于检测环境样品中的低水平草甘膦。通过比较高压灭菌和非高压灭菌土壤泥浆中草甘膦的含量，该测定可用于定量草甘膦降解土壤微生物的活性。
Glyphosate (N-(phosphonomethyl)glycine) is the most widely used herbicide on earth. A simple assay to quantify glyphosate concentrations in environmental samples was developed as part of an interdisciplinary effort linking introductory laboratory courses in chemistry, biology, and microbiology. In this 3 h laboratory experiment, students used UV–vis spectroscopy to quantify glyphosate in prepared unknowns and supernatants from glyphosate-treated soil samples. Regression analysis indicated that the assay is linear up to 20.0 ppm, making it particularly useful for detection of low levels of glyphosate in environmental samples. The assay can be used to quantify the activity of glyphosate-degrading soil microorganisms by comparing glyphosate levels between autoclaved and nonautoclaved soil slurries.