Different alkyl dimethacrylate mediated stearyl methacrylate monoliths for improving separation efficiency of typical alkylbenzenes and proteins
Different alkyl dimethacrylate mediated stearyl methacrylate monoliths for improving separation efficiency of typical alkylbenzenes and proteins
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不同的二甲基丙烯酸烷基酯介导的甲基丙烯酸硬脂酯整料可提高典型烷基苯和蛋白质的分离效率
DOI：
10.1016/j.chroma.2009.01.089
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发表时间：
2009-04-10
影响因子：
4.1
通讯作者：
Wang, Qiuquan
中科院分区：
文献类型：
--
作者：
Xu, Zhendong;Yang, Limin;Wang, Qiuquan
在530 μ m I.D.中制备整料。熔融石英毛细管通过甲基丙烯酸硬脂酯（SMA）与二甲基丙烯酸酯交联剂在含有叔丁基二甲基丙烯酸酯的二元致孔溶剂存在下的原位共聚反应，丁醇和1，4-丁二醇。使用除单体之外的二甲基丙烯酸烷基酯交联剂来调节整料性质，并且，结果，最终整料的疏水性增加（亚甲基选择性α（CH 2）从1.396增加到1.475）通过两个甲基丙烯酸酯单元之间的分子链长度从乙二醇二甲基丙烯酸酯的0.360 nm增加到1.241 nm来观察1.9-壬二醇二甲基丙烯酸酯。此外，交联剂中甲基分支的引入也对整体柱的疏水性有很大影响，其中2-甲基-1，8-辛炔二甲基丙烯酸酯（2-Me-1，8-ODMA）介导的整体柱具有最高的疏水性（α（CH 2）为1.482）和最快的传质动力学（C-term为9.14 ms）。除了对6种模型蛋白质的有效分离外，聚（SMA-co-2-Me-1，8-ODMA）整体柱对烷基苯类化合物的分离性能也有所提高。当以乙腈-水（70：30，v/v）为移动的相，以1 mm/s的典型线速度，硫脲（非截留化合物）和丁苯（截留化合物）的理论塔板数分别达到83 000板/m和52 000板/m。这种对小分子的改进的性能归因于整料中的中孔比例增加和通过选择合适的单体/交联剂对对整料的新剪裁产生的更快的动态传质过程。(C)2009爱思唯尔有限公司版权所有。
Monoliths were prepared in 530 mu m I.D. fused silica capillaries via in situ copolymerization of stearyl methacrylate (SMA) with a dimethacrylate cross-linker in the presence of a binary porogenic solvent containing tert.-butanol and 1,4-butanediol. Alkyl dimethacrylate cross-linkers other than the monomer were used to tune the monolith properties, and, as a result, an increase in the hydrophobicity of the final monoliths (the methylene selectivity alpha(CH2) increased from 1.396 to 1.475) was observed through an increase in the molecular chain length between two methacrylate units from the 0.360 nm of ethylene glycol dimethacrylate to the 1.241 nm of 1.9-nonanediol dimethacrylate. Moreover, the hydrophobicity of the final monoliths was also greatly affected by the methyl group branch in the cross-linkers, among which the 2-methyl-1,8-octanediol dimethacrylate(2-Me-1,8-ODDMA) mediated monolith exhibited the highest hydrophobicity (alpha(CH2), was 1.482) and fastest mass transfer kinetics (C-term was 9.14 ms). Besides the effective separation of six model proteins, the poly(SMA-co-2-Me-1,8-ODDMA) monolith also showed an improved performance in the separation of alkylbenzenes. The theoretical plate numbers reached 83 000 plates/m and 52 000 plates/m for thiourea (nonretained compound) and butylbenzene (retained compound), respectively, when using acetonitrile-water (70:30, v/v) as the mobile phase at a typical linear velocity of 1 mm/s. This improved performance towards small molecules was attributed to an increased mesopore proportion in the monolith and the faster dynamic process of mass transfer arising from novel tailoring of the monolith by choosing a suitable monomer/cross-linker pair. (C) 2009 Elsevier B.V. All rights reserved.