A gadolinium-based magnetic resonance imaging contrast agent for nucleotides sensing
A gadolinium-based magnetic resonance imaging contrast agent for nucleotides sensing
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DOI：
10.1016/j.snb.2017.10.026
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发表时间：
2018-03
影响因子：
8.4
通讯作者：
Jichuan Kong;Zhengkang Liu;Dongxu Cai;Yunchang Fan;Peiran Zhao;Xing Liu;Pengpeng Pu;Lufei Song;Cheng He
中科院分区：
文献类型：
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作者：
Jichuan Kong;Zhengkang Liu;Dongxu Cai;Yunchang Fan;Peiran Zhao;Xing Liu;Pengpeng Pu;Lufei Song;Cheng He
以2-氨基-7-甲基-1，8-萘啶为核苷酸触发位点，通过2，6-二甲基吡啶连接基与钆基造影剂结合，制备了一种新型的磁共振成像探针（Gd-NAPTA），该探针优先响应5 ′-三磷酸鸟苷和5 ′-三磷酸腺嘌呤。由于萘啶与核苷酸碱基之间形成强的多氢键，使得5 ′-三磷酸鸟苷和5 ′-三磷酸腺嘌呤中的磷酸位于合适的位置与镧系离子配位，配位吡啶的取代促进水分子靠近Gd中心，使造影剂的弛豫度增加。Gd-NAPTA的纵向弛豫率（r1）与5 ′-三磷酸鸟苷和5 ′-三磷酸腺嘌呤的浓度呈线性关系。检测限（LOD）约为0.03 mM。Gd-NAPTA的可忽略的细胞毒性和适当的血液循环时间允许磁共振成像的潜在应用体内。
A new magnetic resonance imaging probe(Gd-NAPTA) with 2-amino-7-methyl-1, 8-naphthyridine as the nucleotide triggering site incorporating into gadolinium-based contrast agent through 2,6-dimethylpyridine linker, preferentially responding to guanosine 5′-triphosphate and adenine 5′-triphosphate has been developed. The formation of strong multi-hydrogen bonds between naphthyridine and nucleotide bases made the phosphate in guanosine 5′-triphosphate and adenine 5′-triphosphate positioned on a suitable site to coordinate to the lanthanide ion, such the substitute of the coordinated pyridine promotes the water molecule close to Gd center and the relaxivity increase of the contrast agent. The longitudinal relaxivity(r1) of Gd-NAPTA could linearly respond to the concentration of guanosine 5′-triphosphate and adenine 5′- triphosphate. The limit of detection (LOD) is about 0.03 mM. The negligible cytotoxicity and appropriate blood circulation time of Gd-NAPTA allow potential application of Magnetic Resonance Imaging invivo.