Calcium imaging in Arabidopsis pollen cells using G-CaMP5
Calcium imaging in Arabidopsis pollen cells using G-CaMP5
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使用 G-CaMP5 对拟南芥花粉细胞进行钙成像
DOI：
10.1111/jipb.12642
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发表时间：
2018-09-01
影响因子：
11.4
通讯作者：
Huang, Shanjin
中科院分区：
文献类型：
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作者：
Diao, Min;Qu, Xiaolu;Huang, Shanjin
Ca ~（2+）信号转导与花粉萌发和花粉管生长有关。然而，到目前为止，我们仍然知道的很少，确切地说，在花粉萌发和花粉管生长过程中的各种生理亚细胞过程中的Ca 2+信号联系。鉴于Ca 2+信号传导与细胞溶质浓度和Ca 2+动态密切相关，因此追踪Ca 2+水平的动态变化以解码Ca 2+信号传导至关重要。在这里，我们表明，G-CaMP 5作为一个指标，监测花粉细胞胞质Ca 2+的动态。使用这种探针，我们表明，胞质Ca 2+的变化显着花粉萌发过程中，和，如前所述，Ca 2+形成一个尖端集中的梯度在花粉管中，并经历了振荡的尖端区域在花粉管生长。特别是，使用G-CaMP 5使我们能够捕获响应于各种外源处理的花粉管中胞质Ca 2+浓度（[Ca 2 +]（cyt））的动态变化。我们的数据表明，G-CaMP 5是一个合适的探针，用于监测[Ca 2 +]（cyt）在花粉细胞中的动态。
Calcium (Ca2+) signaling has been implicated in pollen germination and pollen tube growth. To date, however, we still know very little about how exactly Ca2+ signaling links to various physiological subcellular processes during pollen germination and pollen tube growth. Given that Ca2+ signaling is tightly related to the cytosolic concentration and dynamics of Ca2+, it is vital to trace the dynamic changes in Ca2+ levels in order to decode Ca2+ signaling. Here, we demonstrate that G-CaMP5 serves well as an indicator for monitoring cytosolic Ca2+ dynamics in pollen cells. Using this probe, we show that cytosolic Ca2+ changes dramatically during pollen germination, and, as reported previously, Ca2+ forms a tip-focused gradient in the pollen tube and undergoes oscillation in the tip region during pollen tube growth. In particular, using G-CaMP5 allowed us to capture the dynamic changes in the cytosolic Ca2+ concentration ([Ca2+](cyt)) in pollen tubes in response to various exogenous treatments. Our data suggest that G-CaMP5 is a suitable probe for monitoring the dynamics of [Ca2+](cyt) in pollen cells.