Diagnosis of porcine reproductive and respiratory syndrome using infected alveolar macrophages collected from live pigs
Diagnosis of porcine reproductive and respiratory syndrome using infected alveolar macrophages collected from live pigs
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DOI：
10.1016/0378-1135(95)00173-5
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发表时间：
1996-03-01
影响因子：
3.3
通讯作者：
Brockmeier, SL
中科院分区：
文献类型：
--
作者：
Mengeling, WL;Vorwald, AC;Brockmeier, SL
通过检测肺灌洗收集的肺泡巨噬细胞，建立了一种高灵敏度的检测生猪感染猪繁殖与呼吸综合征病毒（PRRSV）的方法。5头猪在10周龄（1头猪）或14周龄（4头猪）时通过口鼻接种或接触PRRSV而暴露。在接触 PRRSV 之前从每头猪身上采集诊断样本（肺泡巨噬细胞和血清）。在接下来的 9 周内，每周收集一次血清，并在第 2 周和第 4-9 周收集肺泡巨噬细胞。血清和肺泡巨噬细和 9 曝光后）。测试肺泡巨噬细胞 PRRSV 的最敏感方法是与 MARC145 细胞共培养。所有猪在接触PRRSV后或由于肺灌洗而出现任何临床症状，并且没有证据表明反复肺灌洗除了引起轻微的、短暂的（轻度充血）组织反应外，还引起任何其他症状。
A highly sensitive method of detecting infection of live pigs with porcine reproductive and respiratory syndrome virus (PRRSV) was developed by testing alveolar macrophages collected by pulmonary lavage, Five pigs were exposed by oronasal inoculation or by contact to PRRSV when they were 10 (1 pig) or 14 weeks (4 pigs) of age. Diagnostic samples (alveolar macrophages and sera) were collected from each pig just before exposure to PRRSV. During the next 9 weeks sera were collected at weekly intervals and alveolar macrophages were collected at weeks 2 and 4-9. Both sera and alveolar macrophages were suitable for detecting early infection, but alveolar macrophages were clearly the better sample after longer intervals, Virus was last isolated from serum at week 4 (from 1 of 5 pigs), whereas it was isolated from the alveolar macrophages of 4 of the 5 pigs at week 4 and from at least 2 pigs at each of the weekly intervals thereafter (i.e. weeks 5, 6, 7, 8, and 9 postexposure). The most sensitive method of testing alveolar macrophages for PRRSV was cocultivation with MARC145 cells. None of the pigs had any clinical signs after exposure to PRRSV or as a result of pulmonary lavage and there was no evidence that repeated pulmonary lavage caused anything other than a mild, transient (mild hyperemia) tissue reaction.