Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: Insights into phylogenetic relationships among the Pipidae
Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: Insights into phylogenetic relationships among the Pipidae
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DOI：
10.1016/j.cbd.2013.10.002
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发表时间：
2013-12-01
影响因子：
3
通讯作者：
King, Jay D.
中科院分区：
文献类型：
--
作者：
Conlon, J. Michael;Prajeep, Manju;King, Jay D.
琵琶科由膜手属、琵琶属、拟膜手属、志留那属和爪蟾属组成，但该科内部的系统发育关系尚不清楚。去甲肾上腺素刺激的皮肤分泌物的肽组学分析，从伪膜手属内的单一物种伪膜手属merlini Chabanaud，1920年，导致13个主机防御肽与抗微生物活性的鉴定。其中2个肽段（Hymenochirin-1 Pa和-1Pb）与来自Hymenochirus boettgeri的Hymenochirin-1B结构相似，8个肽段（Hymenochirin-5 Pa、-5Pb、-5Pc、-5Pd、-5Pe、-5Pf、5 Pg和-5Ph）彼此结构相似且与来自H. boettgeri。两个肽的区别在于一个单一的氨基酸（IKIPSFFRNILKINGKEAVSLM/I AGALKQS），称为pseudhymenochirin-1 Pa和-1Pb，和pseudhymenochirin-2 Pa（GIFPIFAKLLGKVIKVASSLISKGRTE）不类似于宿主防御肽先前分离自pipid蛙。Hymenochirin-5 Pe是分泌物中最丰富的肽，Hymenochirin-1 Pa对金黄色葡萄球菌（MIC = 2.5 μ N）和大肠杆菌（MIC = 10 μ M）最有效。这些数据支持一个密切的系统发育关系之间的Hymenochirus和Pseudhymenochirus是不同的Xenopodinae（非洲爪蟾+志留纪）分支与琵琶姐妹群的所有其他现存的pipids。(C)2013 Elsevier Inc. All rights reserved.
The family Pipidae comprises the genera Hymenochirus, Pipa, Pseudhymenochirus, Silurana, and Xenopus but phylogenetic relationships within the family are unclear. Peptidomic analysis of norepinephrine-stimulated skin secretions from Pseudhymenochirus merlini Chabanaud, 1920, the single species within the genus Pseudhymenochirus, led to identification of 13 host-defense peptides with antimicrobial activity. Two peptides (hymenochirin-1Pa and -1Pb) show structural similarity to hymenochirin-1B from Hymenochirus boettgeri and eight peptides (hymenochirin-5Pa, -5Pb, -5Pc, -5Pd, -5Pe, -5Pf, 5Pg and -5Ph) are structurally similar to each other and to hymenochirin-5B from H. boettgeri. Two peptides differing by a single amino acid (IKIPSFFRNILKINGKEAVSLM/I AGALKQS), termed pseudhymenochirin-1Pa and -1Pb, and pseudhymenochirin-2Pa (GIFPIFAKLLGKVIKVASSLISKGRTE) do not resemble host-defense peptides previously isolated from pipid frogs. Hymenochirin-5Pe was the most abundant peptide in the secretions and hymenochirin-1Pa the most potent against Staphylococcus aureus (MIC = 2.5 mu N) and Escherichia coli (MIC = 10 mu M). The data support a close phylogenetic relationship between Hymenochirus and Pseudhymenochirus that is distinct from the Xenopodinae (Xenopus + Silurana) clade with Pipa sister-group to all other extant pipids. (C) 2013 Elsevier Inc. All rights reserved.