MicroRNA in Alzheimer's disease: an exploratory study in brain, cerebrospinal fluid and plasma.
MicroRNA in Alzheimer's disease: an exploratory study in brain, cerebrospinal fluid and plasma.
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DOI：
10.3109/1354750x.2013.814073
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发表时间：
2013-08
期刊：
影响因子：
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通讯作者：
Leverenz JB
中科院分区：
文献类型：
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作者：
Bekris LM;Lutz F;Montine TJ;Yu CE;Tsuang D;Peskind ER;Leverenz JB
MicroRNA (miRNA) 可能是阿尔茨海默病 (AD) 的潜在生物标志物。本研究的目的是证明人脑或生物液中的 miRNA 根据疾病状态、组织类型、神经炎斑块评分或 Braak 分期的不同而有差异表达。使用阵列对死后大脑 (PMB) miRNA 进行分析，并使用定量 RT-PCR (qRT-PCR) 进行验证。在同一受试者的 PMB、脑脊液和血浆的独立样本中测量了 5 种经过 qRT-PCR 验证的 miRNA。在独立样本中发现血浆 miR-15a 与斑块评分相关。总之，人类生物体液中存在的 miRNA 可以作为 AD 的生物标志物。
MicroRNA (miRNA) may be potential biomarkers of Alzheimer’s disease (AD). The objective of this investigation was to demonstrate that miRNAs in human brain or biofluids are differentially expressed according to disease status, tissue type, neuritic plaque score or Braak stage. Post-mortem brain (PMB) miRNA were profiled using arrays and validated using quantitative RT-PCR (qRT-PCR). Five qRT-PCR-validated miRNAs were measured in an independent sample of PMB, cerebrospinal fluid and plasma from the same subjects. Plasma miR-15a was found to be associated with plaque score in the independent sample. In conclusion, miRNA present in human biofluids may offer utility as biomarkers of AD.