Perovskite ceramic nanoparticles in polymer composites for augmenting bone tissue regeneration
Perovskite ceramic nanoparticles in polymer composites for augmenting bone tissue regeneration
复制标题
DOI：
10.1088/0957-4484/25/48/485101
复制
发表时间：
2014-12-05
期刊：
影响因子：
3.5
通讯作者：
Chatterjee, Kaushik
中科院分区：
文献类型：
--
作者：
Bagchi, Amrit;Meka, Sai Rama Krishna;Chatterjee, Kaushik
人们越来越关注使用纳米粒子作为聚合物基质中的填料来开发模拟骨组织机械、化学和电学特性的生物材料，用于骨科应用。本研究的目的是制备包含三种不同钙钛矿陶瓷纳米颗粒的聚（ε-己内酯）（PCL）纳米复合材料，即钛酸钙（CT）、钛酸锶（ST）和钛酸钡（BT）。复合材料的拉伸强度和模量随着纳米粒子的添加而增加。扫描电子显微镜表明，纳米颗粒的分散度与陶瓷的密度成比例，这反过来又在决定复合材料机械性能的增强方面发挥了重要作用。介电谱显示，与纯 PCL 相比，复合材料的介电常数提高，损耗降低。纳米纤维支架是通过静电纺丝制造的。感应耦合等离子体光发射光谱表明支架释放了少量的Ca+2、Sr+2、Ba+2离子。压力显微镜显示 BT 纳米粒子赋予支架压电特性。体外研究表明，所有复合材料都支持成骨细胞增殖。纳米复合材料上成骨基因的表达按以下顺序增强：PCL/CT>PCL/ST>PCL/BT>PCL。这项研究表明，使用钙钛矿纳米粒子可能是一种有前途的技术，可以为骨组织工程设计更好的聚合物支架。
There is increasing interest in the use of nanoparticles as fillers in polymer matrices to develop biomaterials which mimic the mechanical, chemical and electrical properties of bone tissue for orthopaedic applications. The objective of this study was to prepare poly(epsilon-caprolactone) (PCL) nanocomposites incorporating three different perovskite ceramic nanoparticles, namely, calcium titanate (CT), strontium titanate (ST) and barium titanate (BT). The tensile strength and modulus of the composites increased with the addition of nanoparticles. Scanning electron microscopy indicated that dispersion of the nanoparticles scaled with the density of the ceramics, which in turn played an important role in determining the enhancement in mechanical properties of the composite. Dielectric spectroscopy revealed improved permittivity and reduced losses in the composites when compared to neat PCL. Nanofibrous scaffolds were fabricated via electrospinning. Induction coupled plasma-optical emission spectroscopy indicated the release of small quantities of Ca+2, Sr+2, Ba+2 ions from the scaffolds. Piezo-force microscopy revealed that BT nanoparticles imparted piezoelectric properties to the scaffolds. In vitro studies revealed that all composites support osteoblast proliferation. Expression of osteogenic genes was enhanced on the nanocomposites in the following order: PCL/CT>PCL/ST>PCL/BT>PCL. This study demonstrates that the use of perovskite nanoparticles could be a promising technique to engineer better polymeric scaffolds for bone tissue engineering.