Determination of Breast Cancer Cell Migratory Ability
Determination of Breast Cancer Cell Migratory Ability
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DOI：
10.1007/978-1-4939-3444-7_14
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Tan, Ming
中科院分区：
文献类型：
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作者：
Schmitt, David;Andrews, Joel;Tan, Ming
细胞迁移被定义为单个细胞、细胞片或细胞簇从一个位置到另一个位置的移动(Friedl等人，Int J Dev Biol 48：441-449,2004)。细胞的这种迁移能力对多种正常和病理过程至关重要，包括胚胎发育、伤口愈合、免疫反应和癌症(Leber等人，Int J Oncol34：881-895,2009)。肿瘤细胞的迁移被广泛认为是肿瘤细胞转移到新的部位的重要组成部分，而抑制转移是癌症治疗中的一个重要治疗目标(Horwitz and Webb，Curr Biol 13：R756-759,2003)。因此，观察和量化癌细胞中迁移的能力不仅对基本的癌症生物学至关重要，而且对药物开发尤其重要(Friedl和Gilmour，NAT Rev Mol Cell Biol 10：445-457,2009)。研究人员继续开发测量细胞体外迁移的新技术。本章将讨论两种常用的研究细胞迁移的技术：伤口愈合和Boyden小室迁移试验。
Cell migration is defined as the movement of individual cells, sheets of cells, or clusters of cells from one location to another (Friedl et al., Int J Dev Biol 48: 441-449, 2004). This ability of cells to migrate is critical to a wide variety of normal and pathological processes, including embryonic development, wound healing, immune responses, and cancer (Leber et al., Int J Oncol 34: 881-895, 2009). Migration of tumor cells is widely thought to be an essential component of the metastatic spread of tumor cells to new sites, and inhibiting metastasis is an important therapeutic goal in cancer treatments (Horwitz and Webb, Curr Biol 13: R756-759, 2003). Therefore, the ability to observe and quantify migration in cancer cells is critical not only for basic cancer biology but especially for drug development (Friedl and Gilmour, Nat Rev Mol Cell Biol 10: 445-457, 2009). Researchers continue to develop new techniques for measuring cell migration in vitro. This chapter will discuss two techniques commonly used to study cell migration: wound healing and Boyden chamber migration assays.