3-Nitrotyrosine in Atherosclerotic Blood Vessels
3-Nitrotyrosine in Atherosclerotic Blood Vessels
复制标题
动脉粥样硬化血管中的 3-硝基酪氨酸
DOI：
10.1515/cclm.2003.005
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发表时间：
2003
影响因子：
3.4
通讯作者：
U. Atik
中科院分区：
文献类型：
--
作者：
N. Sucu;A. Ünlü;L. Tamer;B. Aytaçoğlu;B. Ercan;M. Dikmengil;U. Atik
摘要过氧亚硝酸盐在动脉粥样硬化形成中的作用是近年来研究的重点之一。在这里，通过定量测定动脉粥样硬化血管中硝化酪氨酸，3-硝基酪氨酸(3-NT)的水平来研究过氧亚硝酸盐的细胞毒性作用。动脉粥样硬化血管取自接受冠状动脉或外周动脉搭桥术的患者。患者的胸内动脉作为非动脉粥样硬化性对照血管处理。用反相高效液相色谱法测定3-NT，用分光光度法测定血浆亚硝酸盐-硝酸盐水平。动脉粥样硬化组3-NT水平(46.6±23.3nmol/mg蛋白，n=15；P<；0.001)显著高于对照组(15.8±2.5nmol/mg蛋白，n=10)。血管3-NT与血浆硝酸盐水平呈弱相关(r=0.38)。因此，动脉粥样硬化的动脉比非动脉粥样硬化的血管具有更高的3-NT水平。
Abstract The effect of peroxynitrite on the development of atherosclerosis is one of the major foci of recent studies. Here, the cytotoxic effect of peroxynitrite was investigated by quantitatively measuring nitrated tyrosine, 3-nitrotyrosine (3-NT) levels in atherosclerotic blood vessels. Atherosclerotic vessels were obtained from the patients who underwent either coronary artery or peripheric artery bypass surgery. Internal thoracic arteries of the patients were treated as non-atherosclerotic control vessels. 3-NT was measured by reverse-phase HPLC and plasma nitrite-nitrate levels were measured by spectrophotometry. 3-NT levels were significantly elevated in atherosclerotic vessels (46.6±23.3 nmol/mg protein, n = 15; p < 0.001) in comparison to control vessels (15.8±2.5 nmol/mg protein, n = 10). Vessel 3-NT correlated weakly with plasma nitrate levels (r = 0.38). Thus, atherosclerotic arteries have higher 3-NT levels than non-atherosclerotic blood vessels.
DOI：
10.1515/bchm3.1994.375.2.81
发表时间：
1994-02-01
期刊：
BIOLOGICAL CHEMISTRY HOPPE-SEYLER
影响因子：
--
作者：
BECKMANN, JS;YE, YZ;BECKMAN, JS
通讯作者：
BECKMAN, JS