Capillary Electrophoresis Measurements of the Free Solution Mobility for Several Model Polyelectrolyte Systems
Capillary Electrophoresis Measurements of the Free Solution Mobility for Several Model Polyelectrolyte Systems
复制标题
几种模型聚电解质系统自由溶液迁移率的毛细管电泳测量
DOI：
10.1021/ma9903761
复制
发表时间：
1999
期刊：
影响因子：
5.5
通讯作者：
C. Welch
中科院分区：
文献类型：
--
作者：
D. Hoagland;E. Arvanitidou;C. Welch
聚（苯乙烯磺酸）、单链 DNA 和双链 DNA 的自由溶液电泳迁移率通过毛细管电泳在一定范围的离子强度范围内测量，对于聚（苯乙烯磺酸）和单链 DNA，则在一定范围的链长度范围内测量。然后将数据与文献中报告的迁移率和理论预测进行比较。对于低于 0.1 M 的离子强度，毛细管法比以前的技术更加准确和快速；它还提供聚电解质混合物的迁移率值的分布。对于聚（苯乙烯磺酸盐）和 ss-DNA，在低聚物范围内发现了相对于链长的最大自由溶液迁移率；降低离子强度会加剧这种无法解释的现象。在大链极限中，迁移率与链长无关，所有三种聚合物的迁移率对离子强度的依赖性非常相似。这些依赖性只能通过将非线性静电效应纳入...的模型来解释。
The free solution electrophoretic mobilities of poly(styrenesulfonate), ss-DNA, and duplex DNA are measured by capillary electrophoresis across a range of ionic strengths and, for poly(styrenesulfonate) and ss-DNA, across a range of chain lengths. The data are then compared with mobilities reported in the literature and predicted by theory. For ionic strengths below 0.1 M, the capillary method is more accurate and rapid than previous techniques; it also provides a distribution of mobility values for polyelectrolyte mixtures. A maximum of the free solution mobility with respect to chain length is discovered in the oligomer range for both poly(styrenesulfonate) and ss-DNA; lowering ionic strength accentuates this unexplained phenomenon. In the large chain limit, where the mobility is independent of chain length, the ionic strength dependences of mobility for all three polymers are remarkably similar. These dependences can only be explained by models that incorporate nonlinear electrostatic effects into the ...