The effect of age on compensatory and "post-functional hypertrophy" in cross-straited muscle.
The effect of age on compensatory and "post-functional hypertrophy" in cross-straited muscle.
复制标题
年龄对海峡肌肉代偿性和“功能后肥大”的影响。
DOI：
10.1159/000211109
复制
发表时间：
1962
期刊：
影响因子：
--
通讯作者：
E. Gutmann
中科院分区：
文献类型：
--
作者：
Z. Drahota;E. Gutmann
方法以1个月或2岁的大鼠为实验对象进行实验。在测试肌肉代偿反应的实验中，在初次手术时切除一侧的趾长伸肌，同时切断该侧的坐骨神经。 14天后，m。伸肌挖掘。第二面的部分被移除。在这些条件下，肌肉发生代偿性肥大，4-5 个月大的大鼠中总蛋白氮的百分比增加为 12±3.1%（Gutmann，1960）。测定湿重和干重以及总氮。将肌肉在70-80℃真空干燥至恒重。用石油醚提取中性脂肪。干肌肉物质凯氏定氮后，通过康威微扩散技术测定总氮（康威，1940）。在跟踪肌肉刺激后代谢变化的实验中，以300次/分钟的频率直接刺激胫骨前肌6分钟。刺激后2小时和4小时立即切除肌肉并通过缩二脲法测定非胶原蛋白氮的绝对量。核糖核酸的量通过 Schneider 法测定（即 dk，Gutmann 和 Vrbovd，1957）。在另一系列动物中，M. tib。蚂蚁。以120次/分钟的频率刺激一侧的肌肉3分45秒，并在刺激后2小时和4小时立即去除肌肉。糖原水平通过改进的蒽酮方法测定（Klicpera、Drahota 和 dk，1957）。
MethodsThe experiments were carried out in rats aged one month or two years. In the ex periments, in which compensatory reactions in the muscle were tested, the extensor digitorum longus muscle was removed on one side and at the same time the sciatic nerve of this side was cut at an initial operation. 14 days later the m. extensor dig. of the second side was removed. Under these conditions compensatory hypertrophy takes place in the muscle, the percent increase in total protein nitrogen being 12±3.1% in rats 4-5 months old (Gutmann, 1960). Wet and dry weight and total nitrogen were determined. The muscles were dried at 70-80 C in vacuo to constant weight. Neutral fats were extracted with petroleum ether. Total nitrogen was determined after Kjeldahlization of the dry muscle matter by Conway'smicrodiffusion technique (Conway, 1940). In the experiments in which the metabolic changes following stimulation of the muscle were followed, the tibialis anterior muscle was stimulated directly at a frequency of 300/min for 6 min. Immediately, 2 and 4 hours after stimulation the muscles were excised and the absolute amount of noncollagenous protein nitrogen was determined by the biuret method. The amount of ribonucleic acid was determined by Schneider’s method (viz.¿ dk, Gutmann and Vrbovd, 1957). In another series of animals the M. tib. ant. of one side was stimulated at a frequency of 120/min for 3 min 45 sec and the muscles were removed immediately, 2 and 4 hours following stimulation. The levels of glycogen were determined by a modification of the anthrone method (Klicpera, Drahota and¿ dk, 1957).