Integrated transcriptomic and proteomic analyses of a molecular mechanism of radular teeth biomineralization in Cryptochiton stelleri
Integrated transcriptomic and proteomic analyses of a molecular mechanism of radular teeth biomineralization in Cryptochiton stelleri
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DOI：
10.1038/s41598-018-37839-2
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发表时间：
2019-01
影响因子：
4.6
通讯作者：
M. Nemoto;Dongni Ren;Steven A Herrera;Songqin Pan;T. Tamura;K. Inagaki;D. Kisailus
中科院分区：
文献类型：
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作者：
M. Nemoto;Dongni Ren;Steven A Herrera;Songqin Pan;T. Tamura;K. Inagaki;D. Kisailus
已知许多石鳖在其高度矿化和超硬的齿舌齿尖内存款磁铁矿（Fe3O4）。近年来，人们对这些齿冠的超微结构设计和优越的上级力学性能给予了极大的关注，为新型耐磨材料的开发提供了一个有前途的模型。在这里，我们构建了来自C.用于转录组和蛋白质组分析。转录组学分析显示，在非矿化牙齿区域中前20个最高表达的转录本包括编码铁蛋白的转录本，而在矿化牙齿区域中的转录本含有高比例的线粒体呼吸链蛋白。蛋白质组学分析确定了22种蛋白质，在矿化尖特异性表达。这些特异性蛋白质包括一种新的蛋白质，我们称之为齿舌基质蛋白1（RTMP 1），球蛋白，过氧化物酶，抗氧化酶和铁氧化酶蛋白。本研究首次报道了C. stelleri，提供了一个广泛的概述齿舌矿化。这一新的转录组学资源和矿化牙尖的蛋白质组学图谱对于进一步研究石鳖齿舌牙矿化的分子机制具有重要价值。
Many species of chiton are known to deposit magnetite (Fe3O4) within the cusps of their heavily mineralized and ultrahard radular teeth. Recently, much attention has been paid to the ultrastructural design and superior mechanical properties of these radular teeth, providing a promising model for the development of novel abrasion resistant materials. Here, we constructedde novoassembled transcripts from the radular tissue ofC. stellerithat were used for transcriptome and proteome analysis. Transcriptomic analysis revealed that the top 20 most highly expressed transcripts in the non-mineralized teeth region include the transcripts encoding ferritin, while those in the mineralized teeth region contain a high proportion of mitochondrial respiratory chain proteins. Proteomic analysis identified 22 proteins that were specifically expressed in the mineralized cusp. These specific proteins include a novel protein that we term radular teeth matrix protein1 (RTMP1), globins, peroxidasins, antioxidant enzymes and a ferroxidase protein. This study reports the firstde novotranscriptome assembly fromC. stelleri, providing a broad overview of radular teeth mineralization. This new transcriptomic resource and the proteomic profiles of mineralized cusp are valuable for further investigation of the molecular mechanisms of radular teeth mineralization in chitons.