Carboxylesterase-mediated insecticide resistance: Quantitative increase induces broader metabolic resistance than qualitative change
Carboxylesterase-mediated insecticide resistance: Quantitative increase induces broader metabolic resistance than qualitative change
复制标题
羧酸酯酶介导的杀虫剂抗性：量的增加比质的变化引起更广泛的代谢抗性
DOI：
10.1016/j.pestbp.2014.12.016
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发表时间：
2015-06-01
影响因子：
4.7
通讯作者：
Qiao, Chuan-Ling
中科院分区：
文献类型：
--
作者：
Cui, Feng;Li, Mei-Xia;Qiao, Chuan-Ling
Carboxylesterases are mainly involved in the mediation of metabolic resistance of many insects to organophosphate (OP) insecticides. Carboxylesterases underwent two divergent evolutionary events: (1) quantitative mechanism characterized by the overproduction of carboxylesterase protein; and (2) qualitative mechanism caused by changes in enzymatic properties because of mutation from glycine/alanine to aspartate at the 151 site (G/A151D) or from tryptophan to leucine at the 271 site (W271L), following the numbering of Drosophila melanogaster AChE. Qualitative mechanism has been observed in few species. However, whether this carboxylesterase mutation mechanism is prevalent in insects remains unclear. In this study, wild-type, G/A151D and W271L mutant carboxylesterases from Culex pipiens and Aphis gossypii were subjected to germline transformation and then transferred to D. melanogaster. These germlines were ubiquitously expressed as induced by tub-Gal4. In carboxylesterase activity assay, the introduced mutant carboxylesterase did not enhance the overall carboxylesterase activity of flies. This result indicated that G/A151D or W271L mutation disrupted the original activities of the enzyme. Less than 1.5-fold OP resistance was only observed in flies expressing A. gossypii mutant carboxylesterases compared with those expressing A. gossypii wild-type carboxylesterase. However, transgenic flies universally showed low resistance to OP insecticides compared with non-transgenic flies. The flies expressing A. gossypii W271L mutant esterase exhibited 1.5-fold resistance to deltamethrin, a pyrethroid insecticide compared with non-transgenic flies. The present transgenic Drosophila system potentially showed that a quantitative increase in carboxylesterases induced broader resistance of insects to insecticides than a qualitative change. (C) 2014 Elsevier Inc. All rights reserved.
