A batchwise purification procedure of neurofilament proteins.
A batchwise purification procedure of neurofilament proteins.
复制标题
神经丝蛋白的分批纯化程序。
DOI：
10.1016/0003-2697(83)90736-4
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发表时间：
1983
影响因子：
2.9
通讯作者：
Liem,RK
中科院分区：
文献类型：
--
作者：
Tokutake,S;Hutchison,SB;Pachter,JS;Liem,RK
本文报道了一种从牛脊髓中快速纯化神经丝蛋白的方法。可以通过用Triton X-100处理组织，然后通过蔗糖离心来获得粗丝级分。从该粗丝级分中，通过使用羟基磷灰石在8 M尿素中的分批纯化程序来处理蛋白质。用这种方法，在不到3天的时间内从单个牛脊髓中获得约0.5g纯化的神经丝蛋白。
A rapid batchwise purification procedure for neurofilament proteins from bovine spinal cord is described. A crude filament fraction can be obtained by treating the tissue with Triton X-100, followed by centrifugation through sucrose. From this crude filament fraction the protein is processed through a batch purification procedure using hydroxyapatite in 8 m urea. With this procedure, approximately 0.5 g of purified neurofilament protein is obtained from a single bovine spinal cord in less than 3 days.