The cDNA sequence, expression pattern and protein characteristics of mouse protein kinase C-zeta.
The cDNA sequence, expression pattern and protein characteristics of mouse protein kinase C-zeta.
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小鼠蛋白激酶C-zeta的cDNA序列、表达模式和蛋白特征。
DOI：
10.1016/0378-1119(92)90219-f
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发表时间：
1992
期刊：
影响因子：
3.5
通讯作者：
H. Mischak
中科院分区：
文献类型：
--
作者：
J. Goodnight;M. Kazanietz;P. Blumberg;J. Mushinski;H. Mischak
利用逆转录和引物延伸相结合的方法，从小鼠脑中分离到一个2199 bp的编码蛋白激酶C-ζ (PKC-ζ)的互补DNA （cDNA）。预测的PKC-ζ蛋白由592个氨基酸组成，与大鼠PKC-ζ的氨基酸相同99%。用该cDNA探测的Northern blots显示，在小鼠脑中有大量2200个核苷酸（nt）和4200个核苷酸PKC-ζ mrna，数量大致相等。PKC-ζ mRNA在正常肺、肾、睾丸和几种造血肿瘤细胞系中也表达丰富。在所有其他被检测的小鼠组织和细胞系中，至少可以检测到微弱水平的PKC-ζ mrna。在除脑外的其他组织中，PKC-ζ mRNA的数量较少，并且较小的种通常占优势。此外，在这些组织中，两个PKC-ζ mrna似乎比在大脑中发现的两个mrna长约200nt。当cDNA通过杆状病毒表达载体在昆虫细胞中表达时，合成了一个75 kda的蛋白，与其他PKC异构体不同，该蛋白即使在非常高的浓度下也不结合磷酸酯。
A 2199-bp complementary DNA (cDNA) that encodes protein kinase C-ζ (PKC-ζ) has been isolated from mouse brain by a combination of reverse transcription and primer extension. The predicted PKC-ζ protein consists of 592 amino acids which are 99% identical to those of rat PKC-ζ. Northern blots that were probed with this cDNA revealed abundant 2200-nucleotide (nt) and 4200-nt PKC-ζ mRNAs in mouse brain in roughly equal amounts. PKC-ζ mRNA was also abundant in normal lung, kidney, and testes, and in several hemopoietic tumor lines. In all other mouse tissues and cell lines that were examined, at least faint levels of PKC-ζ mRNAs could also be detected. In tissues other than brain, the amount of PKC-ζ mRNA was less, and the smaller species generally predominated. Furthermore, in these tissues, both PKC-ζ mRNAs appear to be approximately 200 nt longer than the two mRNAs found in the brain. When the cDNA is expressed in insect cells via a baculovirus expression vector, a 75-kDa protein is synthesized which, unlike other PKC isoforms, does not bind phorbol ester, even at very high concentrations.
蛋白激酶 C 包含两个佛波酯结合域。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Burns,DJ;Bell,RM
通讯作者：
Bell,RM
DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR