Cloning and characterization of IAR1, a gene required for auxin conjugate sensitivity in Arabidopsis
Cloning and characterization of IAR1, a gene required for auxin conjugate sensitivity in Arabidopsis
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DOI：
10.1105/tpc.12.12.2395
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发表时间：
2000-12-01
期刊：
影响因子：
11.6
通讯作者：
Bartel, B
中科院分区：
文献类型：
--
作者：
Lasswell, J;Rogg, LE;Bartel, B
在高等植物中，大多数吲哚-3-乙酸（IAA）与氨基酸、糖或肽结合，这些结合物参与调节游离激素的浓度。我们确定了iar 1作为拟南芥突变体，它对几种IAA-氨基酸缀合物的抑制作用具有抗性，但对游离IAA仍然敏感。iar 1部分抑制过量产生IAA的突变体的表型，表明IAR 1参与生长素代谢或响应。我们使用位置信息克隆IAR 1，它编码一种新的蛋白质，具有7个预测的跨膜结构域和几个富含组氨酸的区域。IAR 1在其他多细胞生物中有同源物，包括果蝇、线虫和哺乳动物;此外，小鼠同源物KE 4可以在体内功能上替代IAR 1。IAR 1还在结构上类似于金属转运蛋白家族，并具有可检测的序列相似性。我们讨论了几种可能的作用，IAR 1在生长素稳态。
Most indole-3-acetic acid (IAA) in higher plants is conjugated to amino acids, sugars, or peptides, and these conjugates are implicated in regulating the concentration of the free hormone. We identified iar1 as an Arabidopsis mutant that is resistant to the inhibitory effects of several IAA-amino acid conjugates but remains sensitive to free IAA. iar1 partially suppresses phenotypes of a mutant that overproduces IAA, suggesting that IAR1 participates in auxin metabolism or response. We used positional information to clone IAR1, which encodes a novel protein with seven predicted transmembrane domains and several His-rich regions. IAR1 has homologs in other multicellular organisms, including Drosophila, nematodes, and mammals; in addition, the mouse homolog KE4 can functionally substitute for IAR1 in vivo. IAR1 also structurally resembles and has detectable sequence similarity to a family of metal transporters. We discuss several possible roles for IAR1 in auxin homeostasis.