Increased translocation of cardiac protein kinase C β2 accompanies mild cardiac hypertrophy in rats fed saturated fat
Increased translocation of cardiac protein kinase C β2 accompanies mild cardiac hypertrophy in rats fed saturated fat
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DOI：
10.1093/jn/133.2.358
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发表时间：
2003-02-01
影响因子：
4.2
通讯作者：
Kim, S
中科院分区：
文献类型：
--
作者：
Jalili, T;Manning, J;Kim, S
通过蛋白激酶C（PKC）β 2的信号转导可以调节压力超负荷大鼠心肌肥厚。由于PKC β 2可以被脂肪酸和甘油二酯激活，我们假设改变膳食脂肪的水平和类型可能会调节心脏PKC激活，并刺激其他正常大鼠心肌肥大。将雄性Sprague-Dawley大鼠（n = 32）随机分配至低脂肪[10%总能量摄入（TEI）]或高脂肪饮食（40% TEI），以玉米或椰子油作为饱和或不饱和脂肪的来源。经过40天的等能量饮食消费，心脏/体重比略大于饱和脂肪饲料喂养的大鼠相比，不饱和脂肪（P = 0.05）。在所有喂食饱和脂肪饮食的大鼠中也观察到PKC β 2的活化增加，如更大的膜转位所证明的（P < 0.01）。PKC α、β 1和PKC β 2没有变化。这些结果表明，膳食脂肪类型可以改变心脏中PKC β 2的激活，并对心脏产生轻度肥大效应。
Signal transduction through protein kinase C (PKC) beta2 may modulate cardiac hypertrophy in pressure-overloaded rat myocardium. Because PKC beta2 can be activated by fatty acids and diacylglycerol, we hypothesized that altering the level and type of dietary fat might modulate cardiac PKC activation and stimulate hypertrophy in otherwise normal rat myocardium. Male Sprague-Dawley rats (n = 32) were randomly assigned to either a low fat [10% total energy intake (TEI)] or high fat diet (40% TEI) based on corn or coconut oil as a source of saturated or unsaturated fat. After 40 d of isoenergetic diet consumption, the heart/body weight ratio was slightly greater in rats fed saturated fat diets compared with those fed unsaturated fat (P = 0.05). Increased activation of PKC beta2, as evidenced by greater membrane translocation, was also observed in all rats fed saturated fat diets (P < 0.01). PKC alpha, beta1 and epsilon did not change. These results suggest that dietary fat type can alter PKC beta2 activation in the heart, and exert a mild hypertrophic effect on the heart.