Characterization of bacterial communities in hybrid upflow anaerobic sludge blanket (UASB)-membrane bioreactor (MBR) process for berberine antibiotic wastewater treatment
Characterization of bacterial communities in hybrid upflow anaerobic sludge blanket (UASB)-membrane bioreactor (MBR) process for berberine antibiotic wastewater treatment
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DOI：
10.1016/j.biortech.2013.04.077
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发表时间：
2013-08-01
影响因子：
11.4
通讯作者：
Xiao, Shuhu
中科院分区：
文献类型：
--
作者：
Qiu, Guanglei;Song, Yong-hui;Xiao, Shuhu
研究了上流式厌氧污泥床(UASB)-膜生物反应器(MBR)工艺对黄连素类抗生素的生物降解作用。经过118天的运行，小黄碱、COD和NH4+-N的总去除率分别达到99.0%、98.0%和98.0%。利用16S rDNA克隆文库对所建立的细菌群落的详细组成进行了研究。总共检索到400个克隆，并将其归类为186个可操作分类单元(OTU)。UASB以菌丝体和类杆菌为主，变形杆菌，尤其是α-和β-蛋白细菌在MBR中占主导地位。UASB中的梭状芽胞杆菌、真细菌和协生菌以及MBR中的嗜氢菌、固氮菌、鞘氨醇单胞菌、营养单胞菌、志贺氏菌和产碱菌是小连素及其代谢物生物降解的潜在功能物种。两种膜生物反应器的细菌群落组成有显著差异。然而，功能物种的相同功能确保了两个生物反应器具有相当的污染物去除性能。(C)2013爱思唯尔有限公司。保留所有权利。
Biodegradation of berberine antibiotic was investigated in upflow anaerobic sludge blanket (UASB)-membrane bioreactor (MBR) process. After 118 days of operation, 99.0%, 98.0% and 98.0% overall removals of berberine, COD and NH4+-N were achieved, respectively. The detailed composition of the established bacterial communities was studied by using 16S rDNA clone library. Totally, 400 clones were retrieved and grouped into 186 operational taxonomic units (OTUs). UASB was dominated by Firmicutes and Bacteroidetes, while Proteobacteria, especially Alpha- and Beta-proteobacteria were prevalent in the MBRs. Clostridium, Eubacterium and Synergistes in the UASB, as well as Hydrogenophaga, Azoarcus, Sphingomonas, Stenotrophomonas, Shinella and Alcaligenes in the MBRs were identified as potential functional species in biodegradation of berberine and/or its metabolites. The bacterial community compositions in two MBRs were significantly discrepant. However, the identical functions of the functional species ensured the comparable pollutant removal performances in two bioreactors. (C) 2013 Elsevier Ltd. All rights reserved.