Characterization of the mouse Abcc12 gene and its transcript encoding an ATP-binding cassette transporter, an orthologue of human ABCC12
Characterization of the mouse Abcc12 gene and its transcript encoding an ATP-binding cassette transporter, an orthologue of human ABCC12
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DOI：
10.1016/s0378-1119(03)00504-3
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发表时间：
2003-05-22
期刊：
影响因子：
3.5
通讯作者：
Ishikawa, T
中科院分区：
文献类型：
--
作者：
Shimizu, H;Taniguchi, H;Ishikawa, T
我们最近报道了两个新的人类ABC转运蛋白，ABCC11和ABCC12，它们的基因都位于人类染色体16q12.1[Biochem]。生物群落。[中英文摘要]Re.Commun.288(2001)933]。本研究致力于人类ABCC12的小鼠同源基因Abcc12的克隆和鉴定。所克隆的Abcc12基因全长4511个核苷酸，包含一个4101个核苷酸的开放阅读框。推导的多肽由1367个氨基酸组成，与人ABCC12有很高的同源性(84.5%)。小鼠Abcc12基因至少由29个外显子组成，位于小鼠染色体8D3基因座上，该基因与人类染色体16q12.1具有保守的连锁同源性。小鼠的Abcc12基因仅在睾丸的生精小管高水平表达。除了Abcc12转录本外，还检测到了编码短肽(775和687个氨基酸残基)的两个剪接变体。虽然ABCC11和ABCC12的编码基因都位于人类染色体16q12.1上，但在小鼠染色体8D3上没有检测到与人ABCC11对应的小鼠同源基因。(C)2003 Elsevier Science B.V.保留所有权利。
We have recently reported on two novel human ABC transporters, ABCC11 and ABCC12, the genes of which are tandemly located on human chromosome 16q12.1 [Biochem. Biophys. Res. Commun. 288 (2001) 933]. The present study addresses the cloning and characterization of Abcc12, a mouse orthologue of human ABCC12. The cloned Abcc12 cDNA was 4511 bp long, comprising a 4101 bp open reading frame. The deduced peptide consists of 1367 amino acids and exhibits high sequence identity (84.5%) with human ABCC12. The mouse Abcc12 gene consists of at least 29 exons and is located on the mouse chromosome 8D3 locus where conserved linkage homologies have hitherto been identified with human chromosome 16q12.1. The mouse Abcc12 gene was expressed at high levels exclusively in the seminiferous tubules in the testis. In addition to the Abcc12 transcript, two splicing variants encoding short peptides (775 and 687 amino acid residues) were detected. In spite of the genes coding for both ABCC11 and ABCC12 being tandemly located on human chromosome 16q12.1, no putative mouse orthologous gene corresponding to the human ABCC11 was detected at the mouse chromosome 8D3 locus. (C) 2003 Elsevier Science B.V. All rights reserved.