Parthenogenetic activation of porcine oocytes by electric pulse and/or butyrolactone I treatment.
Parthenogenetic activation of porcine oocytes by electric pulse and/or butyrolactone I treatment.
复制标题
通过电脉冲和/或丁内酯 I 处理对猪卵母细胞进行孤雌激活。
DOI：
10.1089/15204559950019843
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
T. Nagai
中科院分区：
文献类型：
--
作者：
A. Dinnyés;Y. Hirao;T. Nagai
本研究的目的是研究特异性细胞周期蛋白依赖激酶抑制剂丁内酯I（BL I）对猪卵母细胞孤雌激活的影响。在实验I中，BL I的有效剂量由激活后卵母细胞中随后的原核形成率来确定。在实验II中，BL I单独或与电脉冲组合进行进一步测试。检查了各种处理诱导激活和孤雌发育的效率。在实验III中，比较了激活的卵母细胞在两种不同培养基中的孤雌发育。检查卵裂率和囊胚发育率，并测定囊胚中的细胞数。我们的研究结果表明，在猪中，BL I的最佳激活剂量为150 μ M;与电脉冲、150 μ M BL I或单独200 μ M BL I相比，电和BL I联合处理导致上级卵裂率（74%，60%，41%，42%，P < 0.05）;激活卵母细胞在mNCSU 37培养液中孤雌发育至囊胚期的比率显著高于Whitten's培养液（59%对5%，P < 0.05），并且所得的第6天胚泡具有更高的细胞数（35.5 +/- 14.1对19.5 +/- 2.5）。该激活方法可用于猪核移植实验和孤雌胚胎的产生。
The goal of the present research was to study the parthenogenetic activation of porcine oocytes following treatment with the specific cyclin-dependent kinase inhibitor butyrolactone I (BL I). In Experiment I, the effective dose of BL I was determined by the rates of the subsequent pronuclear formation in oocytes after the activation. In Experiment II, BL I was further tested alone or in combination with an electric pulse. The efficiency of the various treatments to induce activation and parthenogenetic development was examined. In Experiment III parthenogenetic development of activated oocytes in two different media was compared. Cleavage and blastocyst developmental rates were examined, and number of cells in the blastocysts was determined. Our results indicate that, in pig, the optimal activation dose for BL I was 150 microM; a combined electrical and BL I treatment resulted in superior cleavage rates compared to an electric pulse, 150 microM of BL I, or 200 microM of BL I alone (74%, 60%, 41%, and 42%, respectively; P < 0.05); and the rate of parthenogenetic development of activated oocytes to the blastocyst stage in mNCSU37 medium was significantly higher than that in Whitten's medium (59% vs. 5%, P < 0.05) and the resulting day-6 blastocysts had higher cell numbers (35.5 +/- 14.1 vs. 19.5 +/- 2.5). This activation protocol might be useful in porcine nuclear transfer experiments and for the generation of parthenogenetic fetuses.
影响因子：
8
作者：
M. Kitagawa;H. Higashi;Takahashi Is;T. Okabe;H. Ogino;Y. Taya;S. Hishimura;A. Okuyama
通讯作者：
M. Kitagawa;H. Higashi;Takahashi Is;T. Okabe;H. Ogino;Y. Taya;S. Hishimura;A. Okuyama