Estrogen receptor alpha polymorphisms, estradiol level, and occurrence of atherosclerosis risk factors in healthy postmenopausal women.
Estrogen receptor alpha polymorphisms, estradiol level, and occurrence of atherosclerosis risk factors in healthy postmenopausal women.
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DOI：
10.12659/msm.892831
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发表时间：
2015-04-03
期刊：
影响因子：
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通讯作者：
Walecka I
中科院分区：
文献类型：
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作者：
Bojar I;Gujski M;Raczkiewicz D;Łyszcz R;Owoc J;Walecka I
本研究的目的是分析雌激素受体α基因（Erα）和雌二醇（E2）多态性相互作用与绝经后无心血管疾病的妇女动脉粥样硬化危险因素发生的关系。该研究涵盖了210名妇女，绝经后至少2年，卵泡刺激素浓度为30 mlU/ml，年龄50-60岁，无慢性疾病诊断。在接受检查的女性中，测定了雌二醇、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇和甘油三酯的水平，以及身高、腰围(W)、臀围(R)和动脉高血压。计算BMI和W/H比。采用聚合酶链反应和限制性内切酶（PCR-RFLP）对ER-α多态性进行基因分型。XbaI多态性的等位基因定义为A和G：杂合子AG、野生型GG和纯合子AA。PvuII多态性的等位基因定义为T和C：杂合子TC、纯合子TT和野生型CC。内源性雌二醇浓度和ERα XbaI和PuvII多态性作为独立参数对绝经后妇女的BMI、腰围、W/H比、CHOL、HDL、LDL、TG、LDL/HDL水平无显著影响，对绝经后妇女的心脏收缩和舒张无显著影响。本研究提示ERα XbaI AA多态性可能强化雌二醇对绝经后脂肪组织分布的有益作用；ERα XbaI GG和PuvII TC基因型可增强雌二醇对HDL水平的有益作用；ERα PuvII TT基因型不利于改变绝经后雌二醇浓度与收缩压的关系。
The objective of the study was to analyze the relationship between interaction of polymorphisms in the estrogen receptor alpha gene (Erα) and estradiol (E2), and the occurrence of selected atherosclerosis risk factors in postmenopausal women without the diagnosis of a cardiovascular disease. The study covered 210 women, a minimum of 2 years after menopause, with FSH >30 mlU/ml, aged 50–60 years, with no chronic diseases diagnosed. In the women examined, the levels of estradiol, total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglycerides were determined, as well as height, waist circumference (W), hip circumference (R), and arterial hypertension. The BMI and W/H ratio were calculated. Genotyping of the ER-α polymorphism was performed using a polymerase chain reaction and restriction enzymes (PCR-RFLP). The alleles of the XbaI polymorphism were defined as A and G: heterozygote AG, wild type GG and homozygote AA. The alleles of PvuII polymorphism were defined as T and C: heterozygote TC, homozygote TT, and wild type CC. The concentration of endogenous estradiol and ERα XbaI and PuvII polymorphisms as independent parameters did not significantly affect the BMI, waist circumference, W/H ratio, levels of CHOL, HDL, LDL, TG, or LDL/HDL, nor the systole and diastole in the postmenopausal women in the study. The presented study suggests that ERα XbaI AA polymorphism may intensify the beneficial effect of estradiol on the distribution of fatty tissue after menopause; ERα XbaI GG and PuvII TC genotypes may intensify the beneficial effect of estradiol on HDL level; ERα PuvII TT genotype unfavorably modifies the relation between concentration of estradiol and systolic pressure after menopause.