DEVELOPMENTAL-CHANGES IN HYPOXIA-INDUCED CATECHOLAMINE RELEASE FROM RAT CAROTID-BODY, IN-VITRO
DEVELOPMENTAL-CHANGES IN HYPOXIA-INDUCED CATECHOLAMINE RELEASE FROM RAT CAROTID-BODY, IN-VITRO
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DOI：
10.1113/jphysiol.1994.sp020067
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发表时间：
1994-03-01
影响因子：
5.5
通讯作者：
DOYLE, TP
中科院分区：
文献类型：
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作者：
DONNELLY, DF;DOYLE, TP
1. 使用放置在大鼠颈动脉体中的 Nafion 涂层碳纤维电极在体外研究游离组织儿茶素水平的发育变化。同时，记录来自窦神经的单纤维化学感受器传入活动。检查五个年龄组：1、2、6、10和20-30日龄。2．使用快速扫描伏安法，在暴露于外源性多巴胺期间和灌注低氧盐水期间观察到相似的电流峰。这表明碳纤维电极电流的变化是由于游离组织儿茶酚胺的增加所致。3．与10日龄和20-30日龄大鼠相比，1-6日龄组的基线儿茶酚胺水平显着降低。4．缺氧1分钟后，组织儿茶酚胺峰值浓度在第1天明显低于第2日龄组，且低于第10日龄和20-30日龄大鼠。 5．缺氧期间的峰值神经反应随着年龄的增长而增加，从第1天的4.5+/-0.6Hz增加到第6天的10.5+/-1.6Hz，并在20-30天的大鼠中增加到15.5+/-2.2Hz。6．我们得出的结论是：（1）新生儿期静息游离组织儿茶酚胺水平随着年龄的增长而增加，（2）缺氧导致儿茶酚胺释放增强，（3）产后释放的幅度随着神经活动的增加而增加。
1. Developmental changes in free tissue catecholainine levels were studied using Nafion-coated, carbon fibre electrodes placed in rat carotid bodies, in vitro. Simultaneously, single fibre chemoreceptor afferent activity was recorded from the sinus nerve. Five age groups were examined: 1, 2, 6, 10 and 20-30 days of age.2. Using fast-scan voltammetry, similar current peaks were observed during exposure to exogenous dopamine and during superfusion with hypoxic saline. This suggests that changes in carbon fibre electrode current are due to an increase in free tissue catecholamines.3. Baseline catecholamine levels were significantly less in the 1-6 day age groups compared to 10 day and 20-30 day rats.4. During 1 min of hypoxia the peak concentration of tissue catecholamine was significantly less in the 1 day compared to the 2 day age groups, and these were less than in 10 day and 20-30 day rats.5. Peak nerve response during hypoxia increased with age from 4.5 +/- 0.6 Hz in the 1 day to 10.5 +/- 1.6 Hz in the 6 day and to 15.5 +/- 2.2 Hz in the 20-30 day rats.6. We conclude that (1) resting free tissues catecholamine levels increase with age in the newborn period, (2) hypoxia causes enhanced catecholamine release, and (3) the magnitude of the release increases in the postnatal period as does the nerve activity.