Subtype distribution of Blastocystis spp. isolated from children in Eskisehir, Turkey
Subtype distribution of Blastocystis spp. isolated from children in Eskisehir, Turkey
复制标题
DOI：
10.1016/j.parint.2016.10.008
复制
发表时间：
2017-02-01
影响因子：
1.9
通讯作者：
Dogruman-Al, Funda
中科院分区：
文献类型：
--
作者：
Dogan, Nihal;Aydin, Merve;Dogruman-Al, Funda
芽囊原虫属是人类粪便中最常见的肠道原生生物。囊胚的致病作用仍然存在争议，并已提出囊胚的病理学与其亚型（ST）有关。然而，只有少数研究探讨了儿童芽囊原虫病与亚型之间的关系。本研究旨在调查3 ~ 13岁有或无胃肠道不适的儿童中囊胚的患病率，并确定囊胚的亚型分布。共303个粪便样本从症状（n = 84）和无症状（n = 219）的儿童被纳入研究。采用自然卢戈法、三色染色法和实时荧光PCR法检测囊胚的存在。使用实时PCR方法，发现115个样本为囊胚阳性。使用序列标记位点（STS）引物和PCR对46份样本成功进行了亚型分型。其余69份样本无法分型。最常见的亚型是ST 3（43.4%），其次是STI（26.1%）、ST 4（10.9%）和ST 2（8.7%）。混合亚型5例（10.9%）：ST 1 + ST 3（n = 3），ST 1 + ST 2（n = 1），ST 2 + ST 3（n = 1）。所有样品均不含ST 5、ST 6或S17。在有症状组和无症状组之间，囊胚的阳性率和亚型分布差异无统计学意义（p > 0.05）。据我们所知，这是第一项调查土耳其儿童芽囊原虫亚型分布的研究，结果与土耳其的相关数据一致。(C)2016爱思唯尔爱尔兰有限公司版权所有。
Blastocystis spp. is the most common enteric protist found in human feces. The pathogenic role of Blastocystis remains controversial and it has been suggested that the symptomatology of Blastocystis is associated with its subtypes (ST). However, only few studies have investigated the relationship between the symptomatology and subtypes of Blastocystis in children. This study aimed to investigate the prevalence of Blastocystis in children aged 3 to 13 years with or without gastrointestinal complaints and determine the distribution of the subtypes of Blastocystis. A total of 303 stool samples obtained from symptomatic (n = 84) and asymptomatic (n = 219) children were included in the study. The presence of Blastocystis was investigated using native-lugol examination, trichrome staining and real-time PCR method. Using the real-time PCR method, 115 samples were found positive for Blastocystis. Subtyping was successfully performed on 46 samples using sequenced-tagged site (STS) primers and PCR. The remaining 69 samples could not be subtyped. The most frequently detected subtype was ST3 (43.4%) followed by STI (26.1%), ST4 (10.9%) and ST2 (8.7%). The mixed subtypes were identified in five samples (10.9%) as; ST1 + ST3 (n = 3), STI + ST2 (n = 1) and ST2 + ST3 (n = I). None of the samples had ST5, ST6 or S17. No statistically significant difference was found between the symptomatic and asymptomatic groups in terms of the Blastocystis positivity and the distribution of subtypes (p > 0.05). To our knowledge, this is the first study to investigate the subtype distribution of Blastocystis in children in Turkey and the results are in agreement with the related data available in Turkey. (C) 2016 Elsevier Ireland Ltd. All rights reserved.