Control of oral biofilm formation by an antimicrobial decapeptide
Control of oral biofilm formation by an antimicrobial decapeptide
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DOI：
10.1177/154405910508401215
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发表时间：
2005-12-01
影响因子：
7.6
通讯作者：
Thompson, GA
中科院分区：
文献类型：
--
作者：
Leung, KP;Crowe, TD;Thompson, GA
口腔生物膜是一种混合微生物群落，其不受控制的生长可导致口腔疾病.抗微生物肽代表了对原核生物表现出选择性的替代类别的抗微生物剂。我们想测试合成的十肽抗菌剂KSL对由分离的人类唾液细菌形成的口腔生物膜的发展的影响。我们使用微分干涉对比显微镜，结合双流式细胞系统，以确定KSL对口腔生物膜发育的影响。我们使用活菌计数的减少和共聚焦显微镜来评估KSL对成熟口腔生物膜的杀菌活性。KSL有效地阻止了生物膜的形成。当KSL在表面活性剂的存在下使用时，或在生物膜被机械破坏后，观察到对成熟生物膜的生存能力的显著影响。这项研究表明，KSL可能是一个有用的辅助常规口腔卫生，以防止牙菌斑介导的牙科疾病。
Oral biofilms are mixed- species microbial communities, and their uncontrolled outgrowth can express as oral diseases. Antimicrobial peptides represent alternative classes of antimicrobials that exhibit selectivity for prokaryotes. We wanted to test the effect of a synthetic decapeptide antimicrobial, KSL, on the development of oral biofilms formed by isolated human salivary bacteria. We used differential interference contrast microscopy, coupled with a dual- flow cell system, to determine the effect of KSL on oral biofilm development. We used reductions of viable counts and confocal microscopy to assess the bactericidal activity of KSL on mature oral biofilms. KSL effectively blocked biofilm development. A significant effect on the viability of mature biofilms was observed when KSL was used in the presence of a surface-active agent, or after biofilms were mechanically disrupted. This study shows that KSL may be a useful adjunct for conventional oral hygiene to prevent plaque- mediated dental diseases.