Association between genetic variants in pre-miRNA and colorectal cancer risk in a Chinese population
Association between genetic variants in pre-miRNA and colorectal cancer risk in a Chinese population
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DOI：
10.1007/s00432-013-1456-7
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发表时间：
2013-08-01
影响因子：
3.6
通讯作者：
Zhang, Lin
中科院分区：
文献类型：
--
作者：
Lv, Meili;Dong, Wei;Zhang, Lin
pre-miRNA 中的单核苷酸多态性 (SNP) 可能会改变 microRNA 的表达水平或加工，从而导致癌症发展的易感性。我们假设 pre-miRNA 中的 SNP 可能与结直肠癌 (CRC) 风险相关。我们对 353 名 CRC 患者和 540 名健康对照者的 pre-miRNA 中的四种常见多态性（即 rs11614913、rs3746444、rs2910164 和 rs2292832）进行了基因分型，以研究 SNP 与结直肠癌之间的关联。使用聚合酶链反应限制性片段长度多态性 (PCR-RFLP) 测定法评估 CRC 风险。与 CC 基因型和 C 等位基因相比，rs11614913 CT、TT 基因型和 T 等位基因与 CRC 风险增加相关（CT 与 CC：OR = 7.34，95 % CI 3.76-14.34；TT 与 CC：OR = 13.66， 95% CI 6.76-27.6；T 与 C：OR = 1.99，95% CI 1.63-2.42。有趣的是，以rs2910164 GG基因型作为参考，rs2910164 GC基因型与CRC风险增加相关（OR = 1.49，95 % CI 1.02-2.18），而rs2910164 CC基因型与CRC风险降低相关（OR = 0.58，95 % CI） 0.37-0.93）。与 rs2910164G 等位基因相比，rs2910164 C 等位基因与 CRC 风险降低相关（OR = 0.80，95% CI 0.66-0.97，p = 0.02）。这些发现表明 rs11614913 和 rs2910164 多态性可能与 CRC 的病因有关。
Single-nucleotide polymorphisms (SNPs) in pre-miRNAs may alter microRNA expression levels or processing and then contribute to the susceptibility of cancer development. We hypothesized that SNPs in pre-miRNAs may be associated with the risk of colorectal cancer (CRC).We genotyped four common polymorphisms (i.e., rs11614913, rs3746444, rs2910164, and rs2292832) in pre-miRNAs of 353 CRC patients and 540 healthy controls to investigate the association between the SNPs and the risk of CRC using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.The rs11614913 CT, TT genotypes, and T allele were associated with an increased risk of CRC compared with the CC genotype and C allele (CT vs. CC: OR = 7.34, 95 % CI 3.76-14.34; TT vs. CC: OR = 13.66, 95 % CI 6.76-27.6; T vs. C: OR = 1.99, 95 % CI 1.63-2.42, respectively). Interestingly, using the rs2910164 GG genotype as a reference, the rs2910164 GC genotype was associated with an increased risk of CRC (OR = 1.49, 95 % CI 1.02-2.18), whereas the rs2910164 CC genotype was associated with a decreased risk of CRC (OR = 0.58, 95 % CI 0.37-0.93). When compared with the rs2910164G allele, rs2910164 C allele was associated with a reduced risk of CRC (OR = 0.80, 95 % CI 0.66-0.97, p = 0.02).These findings suggest that rs11614913 and rs2910164 polymorphisms may be associated with the etiology of CRC.