A comparison of DNA extraction methods for food analysis
A comparison of DNA extraction methods for food analysis
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DOI：
10.1016/j.foodcont.2005.08.011
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发表时间：
2007-01-01
期刊：
影响因子：
6
通讯作者：
Tantillo, Giuseppina
中科院分区：
文献类型：
--
作者：
Di Pinto, Angela;Forte, VitoTony;Tantillo, Giuseppina
本文比较了两种用于食品分析的DNA提取和纯化方法，Wizard（R）Magnetic DNA Purification for Food（Promega）和DNeasy（R）Tissue Kit（QIAGEN），从提取效率、DNA纯度和DNA扩增的适用性等方面进行了比较。在这项研究中，两种提取方法的比较突出了Wizard（R）磁性DNA纯化方法对蔬菜基质的效率，而修订后的DNeasy（R）组织试剂盒对复杂和加工基质的效率。(c)2005 Elsevier Ltd.保留所有权利。
In this paper, two DNA extraction and purification procedures for food analysis, Wizard (R) Magnetic DNA Purification for Food (Promega) and DNeasy (R) 'Tissue Kit (QIAGEN), have been compared concerning extraction efficiency, DNA purity and DNA suitability for amplification. The comparison of two extraction methods, in this study, has highlighted the efficiency of the Wizard (R) Magnetic DNA Purification approach for vegetable matrices while the revised DNeasy (R) Tissue Kit for complex and processed matrices. (c) 2005 Elsevier Ltd. All rights reserved.