From the protein to the graph:: How to quantify immunohistochemistry staining of the skin using digital imaging
From the protein to the graph:: How to quantify immunohistochemistry staining of the skin using digital imaging
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DOI：
10.1016/j.jim.2007.12.013
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发表时间：
2008-02-29
影响因子：
2.2
通讯作者：
Kruger-Krasagakis, Sabine
中科院分区：
文献类型：
--
作者：
Kokolakis, Georgios;Panagis, Lambros;Kruger-Krasagakis, Sabine
为了可靠和准确地评估组织中细胞蛋白的表达，需要定量免疫组织化学。皮肤是一个困难的组织自动图像分析，由于其异质性的组成和它的架构。在本研究中，我们使用银屑病皮肤模型，使用数字图像分析比较抗肿瘤坏死因子-α治疗前后p53和bcl-2的表达。用Scion图像分析软件对数字化显微照片进行分析，以获得p53和bcl-2免疫反应细胞面积占研究总面积的比例。方差分析显示，在治疗过程中，p53表达在所有3个表皮层中均显著增加，bcl-2表达在所有3个表皮层中均显著降低（p
Quantitative immunohistochemistry is needed in order to reliably and accurately assess the expression of cellular proteins in tissue. Skin is a difficult tissue for automated image analysis due to its heterogeneous composition and its architecture. In the present study we used a psoriatic skin model to compare the expression of p53 and bcl-2 before and after treatment with anti-tumor necrosis factor-alpha using digital image analysis. Digital photomicrographs were acquired and analyzed with Scion image software in order to obtain the fraction of p53 and bcl-2 immunoreactive cells' area out of the total area investigated. Statistical analysis with ANOVA revealed a significant increase of p53 expression and a decrease of bcl-2 expression in all 3 epidermal layers during the course of therapy (p